1. Insulin improves functional and metabolic recovery of reperfused working rat heart 
Torsten Doenst, MD, R.Todd Richwine, MS, Molly S Bray, PhD, Gary W Goodwin, PhD, O.H Frazier, MD, Heinrich Taegtmeyer, MD, DPhil 
The Annals of Thoracic Surgery 
Volume 67, Issue 6, Pages (June 1999)
DOI: /S (99)

2. Fig 1
Schematic of the experimental protocol and substrates used. Isolated working rat hearts were perfused with Krebs-Henseleit buffer at a preload of 15 cm H2O and an afterload of 100 cm H2O. See Material and Methods for details.
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

3. Fig 2
Rates of glucose uptake before ischemia, during early reperfusion, and during late reperfusion in the group without insulin (control, solid bars, n = 8) and the group with insulin during reperfusion (hatched bars, n = 7). Glucose uptake was assessed by the cumulative release of 3H2O from [2-3H]glucose. Rates were obtained for each interval by linear regression analysis of the respective section of the slope. Note the significant increase in glucose uptake during late reperfusion. Values are mean ± standard error of the mean. (∗p < 0.01 vs before ischemia; +p < 0.01 vs control.) Statistical comparison was by one-way repeated measures analysis of variance, accounting for time and group interaction.
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

4. Fig 3
Rates of glucose oxidation (A) and oleate oxidation (B) before ischemia, during early reperfusion, and during late reperfusion in the group without insulin (control, solid bars, n = 4) and the group with insulin during reperfusion (hatched bars, n = 4). Glucose oxidation was assessed by the cumulative production of 14CO2 from [U-14C]glucose. Oleate oxidation was assessed by the cumulative release of 3H2O from [9,10-3H]oleate. Rates were obtained for each interval by linear regression analysis of the respective section of the slope. Note the significant increase in glucose oxidation during late reperfusion. Values are mean ± standard error of the mean. (∗p < 0.01 vs before ischemia; +p < 0.01 vs control.) Statistical comparison was by one-way repeated measures analysis of variance, accounting for time and group interaction.
The Annals of Thoracic Surgery  , DOI: ( /S (99) )

5. Fig 4
Myocardial glycogen content in the absence (solid bars) or presence (hatched bars) of insulin. Glycogen content was determined before ischemia (n = 4), after 15 minutes of no-flow ischemia (n = 5), after 20 minutes of reperfusion (n = 5 with insulin, n = 5 without insulin), and after 20 minutes of reperfusion plus an additional 20 minutes of reperfusion in the presence of epinephrine (n = 5 with insulin, n = 5 without insulin). See Figure 1 for perfusion protocol. Values are mean ± standard error of the mean. (∗p < 0.01 vs before ischemia; +p < 0.05 vs control.)
The Annals of Thoracic Surgery  , DOI: ( /S (99) )