Presentation is loading. Please wait.

Presentation is loading. Please wait.

The effects of AZD1775 and olaparib on DNA damage response signaling.

Published byBrett Poole Modified over 4 years ago

Similar presentations

Presentation on theme: "The effects of AZD1775 and olaparib on DNA damage response signaling."— Presentation transcript:

1 The effects of AZD1775 and olaparib on DNA damage response signaling.
The effects of AZD1775 and olaparib on DNA damage response signaling. MiaPaCa-2 cells were treated with AZD1775 and olaparib for 1 hour pre- and 6 or 16 hours post-radiation (RT; 6 Gy). At the end of the drug treatment, cells were analyzed by immunoblotting for the indicated proteins. Data are from a single representative experiment (A) or are the mean fold-change in PAR or pCdk1 (Y15) levels relative to control ± SE at 16 hours post-radiation from 3 independent experiments (B and C). Statistical significance (P < 0.05) is indicated versus control* and radiation†. David Karnak et al. Clin Cancer Res 2014;20: ©2014 by American Association for Cancer Research

Download ppt "The effects of AZD1775 and olaparib on DNA damage response signaling."

Similar presentations

Condition MiaPaCa-2 Control Gem (100nm) AZD1775 (200nM) Gem + AZD1775 Panc-1 Control Gem (50nm) AZD1775 (200nM) Gem + AZD1775 Capan-1 Control Gem (50nm)

Condition MiaPaCa-2 Control Gem (100nm) AZD1775 (200nM) Gem + AZD1775 Panc-1 Control Gem (50nm) AZD1775 (200nM) Gem + AZD1775 Capan-1 Control Gem (50nm)
Figure 3. Synergistic effects of the combination of trabectedin and olaparib on poly(ADP-ribosyl)ation and DNA damage in four different breast cancer cell.

Figure 3. Synergistic effects of the combination of trabectedin and olaparib on poly(ADP-ribosyl)ation and DNA damage in four different breast cancer cell.
IDH1 mutant glioma cells are sensitive to TMZ

IDH1 mutant glioma cells are sensitive to TMZ
DNA damage foci in irradiated cells.

DNA damage foci in irradiated cells.
IL-1β stimulates CXCL5 and CXCL8 gene expression and protein secretion in A549 cells in a time- and dose-dependent manner. IL-1β stimulates CXCL5 and CXCL8.

IL-1β stimulates CXCL5 and CXCL8 gene expression and protein secretion in A549 cells in a time- and dose-dependent manner. IL-1β stimulates CXCL5 and CXCL8.
Cytotoxic therapy induces macrophage recruitment, as well as CSF1 and IL-34 mRNA expression. Cytotoxic therapy induces macrophage recruitment, as well.

Cytotoxic therapy induces macrophage recruitment, as well as CSF1 and IL-34 mRNA expression. Cytotoxic therapy induces macrophage recruitment, as well.
A, left: indicated MEFs were transfected as described in the Methods section. A, left: indicated MEFs were transfected as described in the Methods section.

A, left: indicated MEFs were transfected as described in the Methods section. A, left: indicated MEFs were transfected as described in the Methods section.
Pharmacologic inhibition of eIF5A hypusination sensitizes KRas-driven tumor cells to MEK and KRas inhibition. Pharmacologic inhibition of eIF5A hypusination.

Pharmacologic inhibition of eIF5A hypusination sensitizes KRas-driven tumor cells to MEK and KRas inhibition. Pharmacologic inhibition of eIF5A hypusination.
GA blocks HIF activity and reduces HIF target expression.

GA blocks HIF activity and reduces HIF target expression.
Gramicidin A (GA) decreases HIF protein expression in RCC cells.

Gramicidin A (GA) decreases HIF protein expression in RCC cells.
Protein expression profile in a dasatinib-resistant cell line.

Protein expression profile in a dasatinib-resistant cell line.
Heat map of genes for which CR significantly altered expression versus AL. Cluster analysis of genes significantly changed by the CR intervention compared.

Heat map of genes for which CR significantly altered expression versus AL. Cluster analysis of genes significantly changed by the CR intervention compared.
Radiosensitization by combined Wee1 and PARP inhibition.

Radiosensitization by combined Wee1 and PARP inhibition.
Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. A.

Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. A.
DAC treatment activates expression of immunoregulatory genes.

DAC treatment activates expression of immunoregulatory genes.
Pre- and post-vorinostat values of ER-related gene expression using the Oncotype DX 21-gene assay. Pre- and post-vorinostat values of ER-related gene expression.

Pre- and post-vorinostat values of ER-related gene expression using the Oncotype DX 21-gene assay. Pre- and post-vorinostat values of ER-related gene expression.
The effects of IAA and glycyrrhizin (Gc) on proliferation and cell-cycle distribution. The effects of IAA and glycyrrhizin (Gc) on proliferation and cell-cycle.

The effects of IAA and glycyrrhizin (Gc) on proliferation and cell-cycle distribution. The effects of IAA and glycyrrhizin (Gc) on proliferation and cell-cycle.
A, one-step viral growth curves: RT treatment increased the virus yield in MV-CEA–treated U87 cells by up to 2 log as compared with MV-CEA infection only.

A, one-step viral growth curves: RT treatment increased the virus yield in MV-CEA–treated U87 cells by up to 2 log as compared with MV-CEA infection only.
MiR-200c is a PI3K–AKT signaling pathway regulator in CRC

MiR-200c is a PI3K–AKT signaling pathway regulator in CRC
The effect of IAA and glycyrrhizin (Gc) on growth of human melanoma cells. The effect of IAA and glycyrrhizin (Gc) on growth of human melanoma cells. A,

The effect of IAA and glycyrrhizin (Gc) on growth of human melanoma cells. The effect of IAA and glycyrrhizin (Gc) on growth of human melanoma cells. A,

Similar presentations

Ads by Google