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The effects of gastric digestion on codfish allergenicity

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Presentation on theme: "The effects of gastric digestion on codfish allergenicity"— Presentation transcript:

1 The effects of gastric digestion on codfish allergenicity
Eva Untersmayr, MD, Lars K. Poulsen, PhD, Michael H. Platzer, MSc, Mona H. Pedersen, MSc, George Boltz-Nitulescu, PhD, Per Stahl Skov, MD, PhD, Erika Jensen-Jarolim, MD  Journal of Allergy and Clinical Immunology  Volume 115, Issue 2, Pages (February 2005) DOI: /j.jaci Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 Increased pH in SGF experiments hampers codfish allergen digestion. Cod extract (lanes 1) was subjected to digestion experiments for 1 minute with pepsin at pH 1.25 (lanes 2), 2.0 (lanes 3), 2.5 (lanes 4), 2.75 (lanes 5), 3.5 (lanes 6), 4.0 (lanes 7), 4.5 (lanes 8), and 5.0 (lanes 9). A, Coomassie-stained SDS-PAGE. B, IgE immunoblot with a patients' serum pool. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 The dose-dependent inhibitory capacity of codfish extract is reduced by digestion with SGF in a time-dependent manner. Codfish samples were left undigested () or were incubated with pepsin at pH 1.25 for 1 minute (), 15 minutes (), and 1 hour (). The concentrations of proteins applied in RAST inhibition experiments are shown on the x-axis. Uninhibited IgE binding was set at 100%, and the sample values were compared with that value. No inhibition occurred with peanut extract, an irrelevant control antigen (). The mean values of the evaluation with 3 serum samples are presented, and error bars indicate SEMs. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 Dose-dependent HR triggered by digested or undigested codfish extract. The assay was performed with stripped human basophils from a healthy donor passively sensitized with the serum of patient 1 (A), patient 2 (B), patient 3 (C), and a nonallergic control patient (D). Lines (undigested , 1-minute digested , 15-minutes digested , and 1-hour digested , cod extract) indicate the relative percentage of released histamine calculated from the total histamine content of lysed cells, which was set at 100%. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

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