1. FOXO-responsive 3X-IRS promoter activity is reduced in LNAI cells versus LNCaP cells.
FOXO-responsive 3X-IRS promoter activity is reduced in LNAI cells versus LNCaP cells. A. The 3X-IRS promoter, which harbors three insulin response sequences/FOXO response elements (24), was transfected into LNCaP and LNAI cells along with the pRLSV40 (Renilla luciferase). To control for variations in transfection efficiency, the 3X-IRS firefly luciferase activity was normalized to the Renilla luciferase activity from each experimental well (presented in relative luciferase units). The percent reduction in 3X-IRS promoter activity in LNAI cells versus LNCaP cells is noted. B. LNCaP and LNAI cells were transfected with the empty pcDNA control vector or a plasmid vector encoding the wild-type PTEN tumor suppressor, which restrains AKT pathway activity and activates FOXO-mediated transcription (15). Data are presented as the fold induction relative to the values obtained by transfection with the pcDNA empty vector control for each cell line. All transfections were run in triplicate. Columns, mean; bars, SE. These data are representative of more than three independent experiments.
Rebecca L. Lynch et al. Mol Cancer Res 2005;3:
©2005 by American Association for Cancer Research