1. UUKV NSs protein inhibits IFN-β induction at the level of MAVS, whereas HRTV NSs antagonizes at the level of TBK1.
UUKV NSs protein inhibits IFN-β induction at the level of MAVS, whereas HRTV NSs antagonizes at the level of TBK1. (A to E) HEK293T cells were cotransfected with a Firefly luciferase reporter plasmid under the control of an IFN-β promoter and a Renilla luciferase control plasmid in the presence of untagged or V5-tagged UUKV, HRTV, or SFTSV NSs proteins. The cells were stimulated by cotransfection of inducer plasmids encoding FLAG-tagged RIG-I N (A), MAVS (B), TBK1 (C), or IκB kinase ε (IKKε) (D) or by cotransfection of a plasmid expressing untagged IRF3-5D (E). Fold induction was obtained by normalizing the luciferase values to the nonstimulated mock control sample values. Western blots of transfected cell lysates are shown in the lower panels. The data represent results of three independent experiments performed in duplicate (n = 3), presented as fold induction means ± SD. Statistical significance for the comparison of means between groups was determined by one-way ANOVA followed by Dunnett's multiple-comparison post hoc tests. ****, P ≤ ; ***, P ≤ 0.001; **, P ≤ 0.01; *, P ≤ 0.05.
Veronica V. Rezelj et al. mSphere 2017; doi: /mSphere