Presentation is loading. Please wait.

Presentation is loading. Please wait.

Inter-laboratory cohort Prognostication cohort

Published byΤρυφωσα Ιωάννου Modified over 4 years ago

Similar presentations

Presentation on theme: "Inter-laboratory cohort Prognostication cohort"— Presentation transcript:

1 Inter-laboratory cohort Prognostication cohort
Sharp et al; Supplementary Figure S1 Whole PB cohort 277 samples (181 patients) AdnaTestTM Section ; Figure 1 Inter-laboratory cohort CTC cohort IHC cohort Prognostication cohort Excluded: 221 samples No sequential PB sample Excluded: 75 samples No CellSearchTM sample within one month Excluded: 212 samples No tissue biopsy within one month Excluded: 115 samples Duplicate sample No survival data 56 samples (49 patients) Sequential samples Section 3.3; Figure S4 202 samples (136 patients) AdnaTestTM and CellSearchTM within one month Section 3.4; Figure 2 65 samples (58 patients) AdnaTestTM and tissue IHC/NGS within one month Section 3.5; Figure 3 162 samples (162 patients) Unique PB with longest follow up Section 3.6; Figure 4 - AdnaTestTM CTC call and AR-V7/AR-FL quantification - AdnaTestTM CTC call and AR-V7/AR-FL quantification - CellSearchTM CTC count - AdnaTestTM CTC call and AR-V7/AR-FL quantification IHC for AR-FL/AR-V7 protein NGS for AR copy number and mutational status - AdnaTestTM CTC call and AR-V7/AR-FL quantification - Overall survival from PB draw

2 Sharp et al; Supplementary Figure S2

3 Sharp et al; Supplementary Figure S3
Spiked HV blood (500 cells) A B LNCaP95 spiked HV blood LNCaP95 No spike Negative control Positive control Positive Ladder LNCaP 15 cells 250 cells PC3 PSMA PSMA PSA PSA EGFR EGFR Actin Actin C gBlock gene fragments AR-FL amplicon Exon 7-8 AR-V7 amplicon Exon 3-3c gBlock FASTA sequence CAGCCTATTGCGAGAGAGCTGCATCAGTTCACTTTTGACCTGCTAATCAAGTCACACATGGTGAGCGTGGACTTTCCGGAAATGATGGCAGAGATCATCTCTGTGCAAGTGCCCAAGATCCTTTCCCATCTTGTCGTCTTCGGAAATGTTATGAAGCAGGGATGACTCTGGGAGGAAAAATTCCGGGTTGGCAATTGCAAGCATCTCAAAATGACCAGACCCTGAAGAAAGGCTGACTTGCCTCATTCAAA D AR-FL standard curve AR-V7 standard curve Ct value Ct value mRNA expression (copies/ml) mRNA expression (copies/ml)

4 Sharp et al; Supplementary Figure S4

5 Co-occurrence of markers in samples with CTCs detected (136 samples)
Sharp et al; Supplementary Figure S5 Co-occurrence of markers in samples with CTCs detected (136 samples) 136 22 128 70 Actin 19 8 EGFR 67 PSA PSMA

6 CRPC lymph node metastasis (two patients)
Sharp et al; Supplementary Figure S6 AR-FL AR-V7 CRPC lymph node metastasis (two patients)

7 Sharp et al; Supplementary Figure S7
22Rv1* VCaP^ Prostate PC3* AR-FL B 22Rv1* VCaP^ DU145^ PC3* AR-V7

Download ppt "Inter-laboratory cohort Prognostication cohort"

Similar presentations

Interactions Between Vitamin D and Androgen Receptor Signaling in Prostate Cancer Cells Nancy L. Weigel, Ph.D. Baylor College of Medicine.

Interactions Between Vitamin D and Androgen Receptor Signaling in Prostate Cancer Cells Nancy L. Weigel, Ph.D. Baylor College of Medicine.
0 1000 2000 3000 4000 5000 020406080100120 ADT, Estramustine, Dexamethasone Prednisolone Radiotherapy (Months) (ng/mL) PSA Time Patient A Patient B Patient.

ADT, Estramustine, Dexamethasone Prednisolone Radiotherapy (Months) (ng/mL) PSA Time Patient A Patient B Patient.
Blood and Tissue Based Molecular Signatures in Predicting Prostate Cancer Progression Tarek A. Bismar, MD Professor, University of Calgary Departments.

Blood and Tissue Based Molecular Signatures in Predicting Prostate Cancer Progression Tarek A. Bismar, MD Professor, University of Calgary Departments.
Supplementary Figure 2: Representative Kaplan-Meier plots of overall survival considering alterations erbB signaling pathway genes and p53 in lung cancer.

Supplementary Figure 2: Representative Kaplan-Meier plots of overall survival considering alterations erbB signaling pathway genes and p53 in lung cancer.
AR-V7 Splice Variant in Prostate Cancer : Taking Centre Stage

AR-V7 Splice Variant in Prostate Cancer : Taking Centre Stage
Supplementary Figure 1: Correlation analysis of DKC1 mRNA and dyskerin protein quantity in neuroblastoma cell lines.

Supplementary Figure 1: Correlation analysis of DKC1 mRNA and dyskerin protein quantity in neuroblastoma cell lines.
Intermediate Atypical Carcinoma: Novel Histologic Subtype of mCRPC in Patients Resistant to Androgen Receptor Agonists CCO Independent Conference Highlights.

Intermediate Atypical Carcinoma: Novel Histologic Subtype of mCRPC in Patients Resistant to Androgen Receptor Agonists CCO Independent Conference Highlights.
Figure S1. A B C Survival probability (%) P = 0.21 P = 0.37 P = 0.12

Figure S1. A B C Survival probability (%) P = 0.21 P = 0.37 P = 0.12
Frances A Shepherd, MD, Rafael Rosell, MD  Journal of Thoracic Oncology 

Frances A Shepherd, MD, Rafael Rosell, MD  Journal of Thoracic Oncology 
Rupp et al. Supplementary Figure 1: Structure of the human troponin T gene Exon 6 Genomic DNA cDNA from mRNA mutation Exon 9 Exon 10 302bp Parts of genomic.

Rupp et al. Supplementary Figure 1: Structure of the human troponin T gene Exon 6 Genomic DNA cDNA from mRNA mutation Exon 9 Exon bp Parts of genomic.
  TUMOR PD-L1 TIL PD-L1 TUMOR & TIL PD-L1 Age Low High P value R Pearson

  TUMOR PD-L1 TIL PD-L1 TUMOR & TIL PD-L1 Age Low High P value R Pearson
Volume 18, Issue 8, Pages (August 2016)

Volume 18, Issue 8, Pages (August 2016)
Fig. 1. EGFR content as determined by fluorescence in situ hybridization (FISH) and immunohistochemical staining. FISH was performed with the EGFR ( red.

Fig. 1. EGFR content as determined by fluorescence in situ hybridization (FISH) and immunohistochemical staining. FISH was performed with the EGFR ( red.
TBCRC (the translational breast cancer research consortium) 005 Prospective study

TBCRC (the translational breast cancer research consortium) 005 Prospective study
Loss of Hdac3 impairs Ar signaling in mouse prostate tissues and has no effect on apoptosis Loss of Hdac3 impairs Ar signaling in mouse prostate tissues.

Loss of Hdac3 impairs Ar signaling in mouse prostate tissues and has no effect on apoptosis Loss of Hdac3 impairs Ar signaling in mouse prostate tissues.
Summary of genetic alterations in resistant biopsies among patients progressing on ceritinib or alectinib. Summary of genetic alterations in resistant.

Summary of genetic alterations in resistant biopsies among patients progressing on ceritinib or alectinib. Summary of genetic alterations in resistant.
C-myb Transactivates the Human Cyclin A1 Promoter and Induces Cyclin A1 Gene Expression by Carsten Müller, Rong Yang, Gregory Idos, Nicola Tidow, Sven.

C-myb Transactivates the Human Cyclin A1 Promoter and Induces Cyclin A1 Gene Expression by Carsten Müller, Rong Yang, Gregory Idos, Nicola Tidow, Sven.
Lu Chen, PhD, Brienne E. Engel, PhD, Eric A. Welsh, PhD, Sean J

Lu Chen, PhD, Brienne E. Engel, PhD, Eric A. Welsh, PhD, Sean J
Monitoring EGFR mutation status in Non-small cell lung cancer (NSCLC) patients using circulating Tumour DNA (ctDNA). Matthew Smith Molecular Pathology.

Monitoring EGFR mutation status in Non-small cell lung cancer (NSCLC) patients using circulating Tumour DNA (ctDNA). Matthew Smith Molecular Pathology.
Regional lymph nodes and distal extracranial metastases are not a reliable surrogate for actionable mutation in brain metastases. Regional lymph nodes.

Regional lymph nodes and distal extracranial metastases are not a reliable surrogate for actionable mutation in brain metastases. Regional lymph nodes.

Similar presentations

Ads by Google