1. Tumor metastasis is mediated by neutrophils.
Tumor metastasis is mediated by neutrophils. A, total leukocyte (WBC) and neutrophil counts in blood from control noninjected mice or 4 to 5 weeks after implantation with murine mammary carcinoma 4T1 cells. n≥ 9 mice for each group. B, representative picture (top) and average weight (bottom) of spleen from naïve BALB/c (control) or 5 weeks after s.c. implantation of mice with 4T1 cells. n≥ 8 mice for each group. C, 4T1 tumor weight in sham-operated BALB/c mice (control) or splenectomized mice. n≥ 17 for each group. D, number of 4T1 lung metastases (per hematoxylin and eosin–stained section comprising all five lobes) in mice implanted with 4T1 cells in sham-operated BALB/c mice (control) or splenectomized mice. n≥ 12 for each group. E, representative picture of lung lobes from sham surgery or splenectomized mice. Red arrows point to lung metastases. F, D2A1 primary tumor weight 4 to 5 weeks after s.c. implantation of mice with D2A1 cells contralateral to 4T1 cells or Matrigel (control). n≥ 19 for each group. G, D2A1 lung metastases (fold increase vs. D2A1 injected contralaterally to Matrigel) in mice implanted with D2A1 cells contralateral to 4T1 cells or Matrigel (control). n≥ 14 for each group. H, D2A1 lung metastases (fold increase vs. D2A1 injected contralaterally to Matrigel) in sham-operated or splenectomized mice implanted with D2A1 cells contralateral to 4T1 cells. n≥ 8 for each group. I, total leukocyte (WBC) and neutrophil counts in blood from mice 4 to 5 weeks after implantation with D2A1 cells ectopically expressing empty vector (control) or G-CSF. n≥ 9 mice for each group. J, D2A1 lung metastases spontaneously arising in mice bearing primary tumors of D2A1 cells ectopically expressing G-CSF or empty vector (control). n≥ 7 for each group. K, number of D2A1 metastases in lungs of mice lacking primary tumors 2 weeks after i.v. injection of D2A-Tom+ cells. Mice were injected with PBS, or anti-Ly6G antibody 24 hours before i.v. injection of cells. n≥ 7 for each group. *, P < 0.05.
Asaf Spiegel et al. Cancer Discov 2016;6:
©2016 by American Association for Cancer Research