1. Bioluminescence imaging facilitates detection of tumor growth and metastasis in mouse orthotopic xenograft model.
Bioluminescence imaging facilitates detection of tumor growth and metastasis in mouse orthotopic xenograft model. A, PC3-M cells transduced with lentiviruses for firefly luciferase and GFP expression showed high transduction efficiency by comparing phase contrast (left) and fluorescence microscopy (right). Scale bar, 50 μm. B, a luciferase assay of cell dilutions detected as few as 19 cells. C, when PC3-M cells transduced with luciferase virus were implanted orthotopically (3 × 104 cells), tumor growth could be followed in vivo by bioluminescence imaging. D, ex vivo bioluminescence imaging of organs and tissues was used to identify metastases in lung lobes, liver, kidneys, diaphragm, and spleen.
Alexandra Hockla et al. Mol Cancer Res 2012;10:
©2012 by American Association for Cancer Research