1. Functions of myeloid cells.
Functions of myeloid cells. A, Cell sorting strategy. B, T-cell (CD3+) gene expression profile. Cells were sorted as shown in A. Top row, gene expression for Th2 cytokines presented as ΔΔCt normalized to Gapdh; bottom row, gene expression for Th1 cytokines presented as ΔΔCt normalized to Gapdh. Data correspond to the mean ± SEM of 3 individual mice from two experiments. *, P < C, Gene expression analysis of protumorigenic cytokines previously identified as cytokine mediators of MDSC immune suppression. Top, gene expression measured by qPCR on sorted Ly6G; middle, gene expression analysis of Gr1+ cells, from the spleen of 4T1 tumor-bearing mice, treated with conditioned media from Kras/p48 (open bars) or from Kras/p48TGFβRII-KO (closed bars) carcinoma cells; bottom row, gene expression analysis of Gr1+ cells, from the spleen of 4T1 tumor-bearing mice, treated with G-CSF, 20 ng/mL for 6 hours. Data correspond to the mean ± SEM of 3 individual mice from two experiments. **, P < 0.05; *, P < D, T cells (CD3+) were isolated by negative selection from spleen of naïve BALB/c mice, mixed with CD11b cells isolated from pancreatic tissue by using CD11b magnetic microbeads (Miltenyi Biotec), anti–G-CSF was used in concentration 40 ng/mL. Data correspond to the mean ± SEM of three individual mice. *, P < 0.05.
Michael W. Pickup et al. Cancer Immunol Res 2017;5:
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