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Photo-switch Protein 1.

Published byDorothy Burns Modified over 4 years ago

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Presentation on theme: "Photo-switch Protein 1."— Presentation transcript:

1 Photo-switch Protein 1

2 Introduction 2

3 Targeted Protein for Photo-control
DNA binding protein cJun cFos Problem for photo-control Instead Azobenzen Usage Reversibility Repeatability 3

4 Strategy normal mutant 4

5 Pilot experiment prior to cellular experiment (Method for biological section)
5

6 Biochemical Assay suitable inhibitor cJun 6

7 inhibitory test of AFosW in Cell
AP-1 dependent promoter-GFP CMV-promoter -RFP AP-1 dependent promoter-GFP AP-1 dependent promoter-GFP CMV-promoter -RFP CMV-promoter -RFP GFP is not expressed. + AFosW Inhibition by AFosW 7

8 Results of Photoregulation of AP-1 activity in living cells
azobenzen-based cross-linker 8

9 Azo-compound cross-linked peptide, XAFosW (CD spectrum)
cross linking positions 365 nm irradiation 9

10 Azo-compound cross-linked peptide, XAFosW (Binding Assay)
Inhibition for DNA binding 10

11 Photoswitchable inhibitory effect in living cell (Checking Activity) .
activity ratio luciferase -galactosidase 11

12 Conclusion 12

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