1. GENETICS UNIT FIVE DAY 1

2. OPENER If you did your Spring Break Genetics Review HW, take it out and raise your hand in the NEXT 30 SECONDS.

3. AGENDA Three Questions—Genetics Review Topics for this Unit First Learning Target Notes/Modeling/Summary: The Operon Model of Prokaryotic Regulation of Gene Expression

4. THREE QUESTIONS 1.What is the function of DNA? How does the structure of the DNA molecule allow DNA to do its job? 2.How is the information stored in DNA accessed and used? 3.How is the structure of the DNA molecule related to the structure of protein molecules? Why do we care about proteins? Tables 1-2: Question 1 Tables 3-5: Question 2 Tables 7-8: Question 3

5. ANSWERS 1.DNA stores the information cells need to make the proteins. This information is stored in the order in which the smaller parts of the DNA molecule (the nucleotides) are arranged (like the order of letters in a word give a word meaning). 2.The information in DNA is copied into RNA, in a process called transcription. This information is used by ribosomes to make proteins, in a process called translation. 3.The ‘genetic code’ contained in DNA and RNA is based on groups of three nucleotides, called codons. Each codon “codes” for a different amino acid in a protein.

6. CENTRAL FRAMEWORK OF MOLECULAR BIOLOGY DNARNAProteinTrait Transcription Translation The “expression” of genes

7. REGULATION OF GENE EXPRESSION CANCER AND THE CELL CYCLE TWO TOPICS

8. LEARNING TARGET (S) LEVEL 3: I CAN EXPLAIN HOW GENE EXPRESSION IS REGULATED IN PROKARYOTIC CELLS LEVEL 4: I CAN COMPARE AND CONTRAST THE REGULATION OF PROKARYOTIC AND EUKARYOTIC GENE EXPRESSION

9. BUT FIRST...

10. Insulin Human Growth Hormone Interferon Biofuel?

12. WHY REGULATE? WHY DO CELLS NEED TO CONTROL WHAT GENES ARE TURNED ON?

13. DNA

17. OPERON Simplest way to control gene expression? Stop mRNA transcription Prokaryotes use operons to control transcription An operon is a piece of a DNA molecule that includes: Genes DNA sequences involved in controlling gene expression: on/off switches On/off switch Promoter Operator Proteins also part of switch: Repressors Co-repressors (activators OR inhibitors)

19. TWO BASIC TYPES The INDUCIBLE OPERON Normal state: switched OFF Transcription STARTS when co-repressor binds to repressor (inactivating it) Lac operon: regulates production of proteins needed for digesting lactose The REPRESSIBLE OPERON Normal stated: switched ON Transcription STOPS when co-repressor binds to repressor (activating it) Trp operon: regulates production of proteins needed to make tryptophan (an amino acid)

20. INDUCIBLE HOW IT WORKS... Gene PromoterOperator RNA Polymerase Gene OFF! Gene ON! Pac Man=Repressor Orange Square=Co-Repressor mRNA BINDING BLOCKED!!

21. REPRESSIBLE HOW IT WORKS... Gene PromoterOperator RNA Polymerase Gene On! Gene OFF! Pac Man=Repressor Orange Square=Co-Repressor mRNA BINDING BLOCKED!!

22. THE LAC OPERON ANIMATION STYLE

23. POWER! TWO TOOLS: 1) ON/OFF SWITCH 2) GENE SPLICING

24. PRACTICE Use your models to practice explaining to a partner how BOTH inducible and repressible operons work Take turns As you explain how operons work, use the: operator, promoter, repressor, co-repressor, RNA polymerase, transcription and gene.

25. REPETITIONS Step One: Go through your notes and highlight each WORD you need to know to describe how operons function Step Two: Summarize your notes. Use all the words we have identified. This summary is a first draft of the introduction to your lab report.

26. READ THE LAB HANDOUT!! HOMEWORK