1. Gene delivery of ChABC leads to reduced neuronal death associated with lower levels of apoptosis at 1 week postinjury.
Gene delivery of ChABC leads to reduced neuronal death associated with lower levels of apoptosis at 1 week postinjury. A, B, Serial images of NeuN (neurons, green) immunoreactivity throughout the rostrocaudal axis showing greater survival of spinal neurons at 1 week postinjury following LV-ChABC treatment, compared with LV-GFP treatment. C, D, Cleaved caspase-3 immunoreactivity in the lesion epicenter reveals a clear reduction in the apoptotic marker at 1 week postinjury following LV-ChABC treatment, compared with LV-GFP treatment. E, Quantification of total NeuN-positive cells counted in serial sections from 1 mm rostral to 1 mm caudal to the epicenter revealed a significant preservation of spinal neurons following LV-ChABC treatment, compared with contusion only and LV-GFP treatment; *p < 0.05, unpaired two-tailed Student's t test. F, Quantification of cleaved caspase-3 activity in the lesion epicenter revealed reduced levels of apoptosis following LV-ChABC treatment, compared with LV-GFP controls; *p < 0.05, unpaired two-tailed Student's t test. Scale bar: (in C) A–D, 500 μm.
Katalin Bartus et al. J. Neurosci. 2014;34:
©2014 by Society for Neuroscience