1. Fig. 5 Hypoxic tumors from obese mice associate with increased production of IL-6 by adipocytes and myeloid cells.
Hypoxic tumors from obese mice associate with increased production of IL-6 by adipocytes and myeloid cells. (A) mRNA expression (normalized to a panel of housekeeping genes) of proinflammatory cytokines and proangiogenic growth factors in E0771 tumors from lean and obese animals treated with B20. Four samples per group were pooled in each array plate used. Genes that increased more than about twofold are included (in addition to VEGF family genes). (B) Protein expression of IL-6 in E0771 tumors from lean and obese animals treated with B20. Significant differences using t test are indicated, *P < Data are shown as individual values plus box plots with minimum, maximum, and median values. (C) Immunohistochemistry indicating IL-6 expression in adipocyte-rich areas (arrows point to adipocytes). (D) Immunofluorescence showing IL-6 expression and hypoxic (GLUT-1+) adipocyte-rich areas (arrow points to adipocytes). (E) Colocalization of IL-6 with the myeloid marker CD11b (arrow points to adipocytes). (F) Colocalization of macrophages (F4/80+ cells) with IL-6 in tumors (arrows point to adipocytes). (G) Colocalization of macrophages (F4/80+ cells) with adipocytes in hypoxic (CA-IX–positive) areas. Scale bar, 400 μm. (H) E0771 BC cells express IL-6R and the IL-6 signal-transducing subunit gp130. (I) Immunofluorescence showing tumor expression of p-STAT3 and macrophages infiltrating adipocyte-rich areas (arrow points to an adipocyte). Scale bar, 100 μm.
Joao Incio et al., Sci Transl Med 2018;10:eaag0945
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