1. Loss of Cav1 function does not disrupt formation of dual projecting CPN/FPN axonal projections at P8.
Loss of Cav1 function does not disrupt formation of dual projecting CPN/FPN axonal projections at P8. A–F, CPN (green) were retrogradely labeled from contralateral somatosensory cortex, and FPN (red) from ipsilateral premotor cortex in Cav1-null mice (D–F; n = 5) and WT littermates (A–C; n = 5). A, D, CPN; B, E, FPN; and C, F, CPN/FPN form in the absence of Cav1 function. G, H, The number of dual projecting CPN/FPN (G) and total FPN (H) were counted for two medio-lateral regions (S1 and S2). There is no significant difference between the two genotypes (n = 5 WT and n = 5 Cav1-nulls). I, The percentage of total labeled FPN that have a dual callosal projection was also calculated for two medio-lateral regions (S1 and S2). There is no significant difference between the two genotypes (n = 5 WT and 5 Cav1-null). Error bars denote SEM. FPN, ipsilateral FPN. Scale bars: 500 μm (A–F) and 100 μm (A’–F’, A’’–F’’).
Jessica L. MacDonald et al. eNeuro 2018;5:ENEURO
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