1. FGFR1 and βKlotho are expressed in the hypothalamus of mice.
FGFR1 and βKlotho are expressed in the hypothalamus of mice. The abundance of FGFR1 mRNA and protein (A) and βKlotho mRNA and protein (C) in liver, white adipose tissue (WAT), and hypothalamus (hypo) in 12-week-old male C57BL/6J mice, as determined by real-time PCR and Western blot analysis, respectively. Representative images of mouse hypothalamic slices immunostained for FGFR1 (B), βKlotho (D), and CRH (B). Note that the merged image shows the colocalization between FGFR1 and CRH (B), and the specificity of anti-CRH antibody (catalog no. ab11133, Abcam) was validated by using the CRH blocking peptide (Sigma-Aldrich, catalog no. C3042) to neutralize the antibody. No positive fluorescent signal was detected when the hypothalamic slices were coincubated with anti-CRH antibody and blocking peptides (data not shown). Representative Western blotting analysis and immunostained figures from three independent cohorts are shown; n = 6.
Qingning Liang et al. Diabetes 2014;63:
©2014 by American Diabetes Association