1. From: Mitochondrial Localization and Ocular Expression of Mutant Opa3 in a Mouse Model of 3-Methylglutaconicaciduria Type III
Invest. Ophthalmol. Vis. Sci ;52(7): doi: /iovs
Figure Legend:
Opa3 mRNA is expressed from early development, and the protein is present in all adult tissues tested. (A) RT-PCR showing expression of Opa3 during embryonic development in whole embryos from E8 to E10, in head and body subsets of embryos from E13 to birth, and in adult (P30) lens, retina, and optic nerve. (B) Western blot of Opa3 protein expression in a panel of mouse tissue lysates (skeletal muscle, kidney, heart, liver, lung, brain, retina and lens). In the blot shown, rabbit anti-Opa3 antibody was used and detects bands at 20 and/or 40 kDa (top). Bottom: VDAC/Porin as a mitochondrial marker. (C) Western blot of Opa3 protein expressed in human embryonic kidney cells (HEK) and in a panel of mouse tissue lysates (kidney, liver mitochondrial fraction, liver, lung, brain, retina, and lens), using increased reducing agent in sample buffer. Rabbit anti-Opa3 antibody detected bands at 20 and 40 kDa (top). (Composite image from more than one gel.)
Date of download: 12/18/2017
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