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Fig. 2. Sufficient rate of recombination in embryonic NSCs and NPCs of Emx1cre knock-in mice.(A) Breeding schemes to generate mice with the Emx1cre locus.

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Presentation on theme: "Fig. 2. Sufficient rate of recombination in embryonic NSCs and NPCs of Emx1cre knock-in mice.(A) Breeding schemes to generate mice with the Emx1cre locus."— Presentation transcript:

1 Fig. 2. Sufficient rate of recombination in embryonic NSCs and NPCs of Emx1cre knock-in mice.(A) Breeding schemes to generate mice with the Emx1cre locus on the tdTom reporter background. Sufficient rate of recombination in embryonic NSCs and NPCs of Emx1cre knock-in mice.(A) Breeding schemes to generate mice with the Emx1cre locus on the tdTom reporter background. (B) tdTom reporter expression (red) in the E12.5 and E14.5 Emx1cre brains. Robust tdTom expression was detected in the entire cortex at both E12.5 and E14.5. (C) Analysis of cre expression at ages corresponding to sections in B, using a cre-specific antibody (green), in combination with tdTom and DAPI (blue). Cre(−) controls were obtained from brains of mice negative for Emx1cre, but genetically positive for tdTom reporter. (D) Emx1cre recombination in neural progenitors of E14.5 cortex (high magnification for the box region indicated in 2B). Emx1cre mediated tdTom expression (red) was found in the entire cortex including the proliferative VZ and SVZ. High magnification of the VZ regions (box) revealed robust co-localization of PH3+ mitotic NSCs (green) with tdTom (red). Scale bars: B, 300 µm; C, 30 µm; D, low mag (left), 20 µm; high mag (right panels), 10 µm. Huixuan Liang et al. Biology Open 2012;bio © Published by The Company of Biologists Ltd

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