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Persistence of the IgE repertoire in birch pollen allergy

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Presentation on theme: "Persistence of the IgE repertoire in birch pollen allergy"— Presentation transcript:

1 Persistence of the IgE repertoire in birch pollen allergy
Manuel Otte, PhD, Vera Mahler, MD, Andrea Kerpes, Oliver Pabst, PhD, David Voehringer, PhD  Journal of Allergy and Clinical Immunology  Volume 137, Issue 6, Pages e8 (June 2016) DOI: /j.jaci Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Analysis of immunoglobulin repertoire overlap between isotypes and between birch pollen seasons. A, CDRH3 sequence overlap between indicated immunoglobulin isotypes in S_2013 and S_2014 displayed by Morisita-Horn index. B, CDRH3 sequence overlap within indicated isotypes between S_2013 and S_2014. Bars show the mean + SD from 3 patients. C, Frequency (red, >1%; orange, 0.1% to 1%; yellow, 0.01% to 0.1%) of particular CDRH3 sequences in the IgE repertoires of indicated patients and seasons (S13, season 2013; OS13, off-season 2013; S14, season 2014). Each row in each grouped set of 3 columns reflects one particular CDRH3 sequence. Heat maps are ordered by the 50 most abundant CDRH3 sequences in S13 (left), OS13 (middle), and S14 (right). Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Analysis of SMs in VH genes of different isotypes. A, Number and frequency of nonsilent mutations among productive sequences of indicated isotypes in S_2013 and S_2014. B, Distribution of silent and nonsilent mutations among indicated regions of the VH genes in the whole IgE repertoire. Bars show the mean + SD from 3 patients and are calculated from all productive IgE sequences. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 Immunoblot with total birch pollen extract. Serum samples from the 3 patients and 1 healthy control were analyzed for binding to SDS-PAGE–separated proteins from whole birch pollen extract as described in the Methods section. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E2 Spectrum of allergic sensitization revealed by ImmunoCAP Immuno Solid-phase Allergen Chip Multiplex Chip analysis. White, <0.3 (kU IgE/L); yellow, (kU IgE/L); orange, 5-25 (kU IgE/L); red, >25 (kU IgE/L). Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E3 IgE repertoire complexity in comparison to other isotypes. Number of total sequences (A), frequency of productive sequences (B), and repertoire complexity (richness) measured by the number of different CDRH3 sequences in 1000 randomly picked sequences (C) from indicated immunoglobulin isotypes in birch pollen S_2013 and S_2014. Bars show the mean + SD from 3 patients. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E4 VH, DH, and JH usage by IgE and other isotypes. All productive sequences were analyzed for usage of indicated VH, DH, and JH families in birch pollen S_2013 and S_2014. Bars show the mean + SD from 3 patients. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E5 Distribution and persistence of somatic mutation between seasons. A total of 25 randomly picked IgE sequences that shared the same CDRH3 region in S_2013 and S_2014 were used for comparison of the localization of SMs across the VH genes. The name of the germline VH gene is given for each group of analyzed sequences. Identical mutations in S_2013 and S_2014 are marked in red color. The position of additional mutations is indicated by black dots. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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