1. Differential secretion profiles of C. elegans TTR models.
Differential secretion profiles of C. elegans TTR models. (A) Representative confocal IF images of adult day-1 animals stained with a polyclonal antibody against TTR (green) and phalloidin (F-actin; red). The panels at the far right show maximal intensity projections of whole animals. Insets in white boxes show enlargements of head regions in the adjacent column on the left. Arrowheads point to muscle cells; arrows point to the body cavity. The dotted line in the control F-actin panel outlines the contour of a single body-wall muscle cell. Insets in orange boxes show enlargements of aggregates. Insets in cyan boxes show enlargements of cell-surface TTR staining. (Scale bars: 50 μm.) (B) Schematic diagram of the experiment in which worms were fed CMPD5 to detect TTR–(CMPD5)2 conjugates. The black box shows coelomocytes enlarged in C. The pharynx is shown in green. (C) Enlargements showing coelomocytes. Arrows point to coelomocytes. Control, n = 11; WT, n = 12; V30M, n = 15; T119M, n = 21; D18G, n = 23. (Scale bar: 10 μm.) DIC, differential interference contrast microscopy. See also SI Appendix, Fig. S2.
Kayalvizhi Madhivanan et al. PNAS 2018;115:33:E7710-E7719
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