1. Identification and characterization of a xap5 mutant in Chlamydomonas.
Identification and characterization of a xap5 mutant in Chlamydomonas. (A) Motile cells of the wild-type (WT) and rescued (xap5::XAP5-HA) strains were observed as long, winding tracks; conversely, palmelloid cells of af1-x were presented as bright focus. (Scale bar, 20 μm.) (B) Differential interference contrast (DIC) images of wild-type, af1-x, and rescued cells. Palmelloid af1-x cells were incubated with autolysin. (Scale bar, 5 μm.) (C) Immunofluorescence micrographs of wild-type and af1-x flagella labeled with acetylated tubulin antibodies after treatment with autolysin. (Scale bar, 10 μm.) (D) Flagellar length distribution of wild-type and af1-x cells after treatment with autolysin (n = 500). (E) Schematic view of the XAP5 gene; the foreign DNA insertion site in af1-x and the domain structure of the XAP5 protein are indicated.
Lili Li et al. PNAS 2018;115:17:
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