1. Pou5f1 is required for proper developmental timing of gene expression.
Pou5f1 is required for proper developmental timing of gene expression. (A) Microarray expression profiles in WT (white squares) and MZ (black triangles) of Cluster A genes downregulated in MZ. (B) Diagram of selected Cluster A (downregulated in MZ, black font) and DE (upregulated in MZ, white font) genes organized by their normal time of activation in WT. In WT, Cluster A genes start to be expressed at 3–8 hpf, whereas in MZ their expression during these stages is reduced or absent. In contrast, in WT most Cluster DE genes start to be expressed at >8 hpf, and are involved in tissue specification and differentiation. In MZ, expression of Cluster DE genes is prematurely activated at gastrula stages. (C) Microarray expression profiles in WT (white squares) and MZ (black triangles) of Cluster DE genes prematurely activated in MZ. (A, C) Values were normalized to maximal expression (100). Error bars show s.e.m. of two probes (foxD3, klf2b, klf4, nr2f1) or of three biological replicates for one probe. (D) Whole‐mount in situ hybridization, lateral view with animal pole at top, except where animal pole view is indicated. Cluster DE genes with different activation time and spatial pattern in MZ: pax6a prematurely appears after 7 hpf in the neuroectodermal region of MZ, nr2f1 is ubiquitously upregulated already at sphere (4 hpf).
Daria Onichtchouk et al. Mol Syst Biol 2010;6:354
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