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Functional metabolic rearrangements in chloramphenicol‐resistant populations Functional metabolic rearrangements in chloramphenicol‐resistant populations.

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Presentation on theme: "Functional metabolic rearrangements in chloramphenicol‐resistant populations Functional metabolic rearrangements in chloramphenicol‐resistant populations."— Presentation transcript:

1 Functional metabolic rearrangements in chloramphenicol‐resistant populations
Functional metabolic rearrangements in chloramphenicol‐resistant populations List of EMCs predicted in chloramphenicol‐glucose‐evolved mutants. Reactions are grouped on the basis of their topological distance, by means of the minimum number of connecting reactions on the metabolic network. For EMCs predicted in chloramphenicol‐glucose, filled marks on the right‐hand side highlight whether the same EMC was found also in the other evolved populations.Experimentally measured fluxes exclusively in evolved populations grown in glucose minimal medium. Absolute glucose consumption is reported in mmol/gDW/h, growth rate in h−1. Acetate secretion and oxygen consumption rates are reported as a percentage relative to glucose uptake. Data have been grouped according to the selective pressure and for each group. The tops and bottoms of each box are the 25th and 75th percentiles of the samples, respectively, while the red line in the middle of each box is the sample median (Dataset EV5 contains mean ± SD of three biological replicates).Genetic changes identified by whole‐genome sequencing. Genetic changes identified in at least two out of the four lineages evolved under the same selective pressure are retained. The bipartite graph links selective pressures (i.e. chloramphenicol‐glucose and chloramphenicol‐acetate) to mutated genes. Arrow size represents the number of lineages with at least one sequence change in the corresponding gene or its upstream regulatory sequence.Western blot analysis monitoring the AcrB protein abundance across antibiotic‐resistant populations evolved in glucose, wild type and populations evolved in glucose and acetate without antibiotics. Asterisks indicate statistically significant difference from wild type E. coli (**P < 0.01 from t‐test analysis). Data are the mean ± SD of two replicates. One of the Western blots is shown. Mattia Zampieri et al. Mol Syst Biol 2017;13:917 © as stated in the article, figure or figure legend

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