1. Theoretical Background
Dayun Yu
Department of Physics
Kangwon National University
Surface Plasmon Resonance (SPR)
Optical principle.
Dispersion relation
Advantage of the surface plasmon resonance
Mass Transport in Boundary Layer
Effect of the mass transport
Benefit of the mass transport

2. = Optical Principle (SPR) : Condition of Resonance Light (ω)
angle
Reflectivity
Light (ω)
2D-detector
array
Surface Plasmon wave vector
Evanescent wave vector
Condition of
Resonance = :

3. Dispersion Relation K w w0
Theory of SPR
Ksp= w/c x {(emr x es)/(emr + es)}1/2
Ks=w/c x (es)1/2
Kretschmann’s prism method
Kev=Kp x sinq=w/c x (ep)1/2 x sinq
Kev=Ksp
(Resonance point : w0)
qsp=sin x [(emx es)/{ep(emr + es)}]1/2
Fig. Dispersion of evanescent wave vector(kev) and surface plasmon wave vector(ksp)

4. Advantage of the SPR Monitor binding rapidly Measurement in real time
Process experiment without labels

5. Effect of Mass Transport
Gold surface
Flow
Mass transport
Sample (analyte)
Ligand
Diffusion
} Binding
Binding of analyte onto the gold surface becomes more dominant than the diffusion of analyte near the surface.

6. Benefit of the Mass Transport
Determine the active concentration and flow rate of analyte
The investigation of fluidics

7. Summery
Surface Plasmon Resonance - Detection of binding is based on the SPR phenomenon - Advantage of SPR # Monitor binding rapidly # Measurement in real time # Process experiment without labels
Mass transport in boundary layer - Mass transport occur that binding of analyte onto the gold surface becomes more dominant than the diffusion of analyte near the surface - Benefit of mass transport # Determine the active concentration and flow rate of the analyte # Investigation of the fluidics