1. (A) CDK2 and p53 gene expression levels, as quantitated by real-time quantitative PCR with SYBR Green master mix (Stratagene, La Jolla, Calif.).
(A) CDK2 and p53 gene expression levels, as quantitated by real-time quantitative PCR with SYBR Green master mix (Stratagene, La Jolla, Calif.). The relative expression of the mRNA species was calculated by the comparative CT method, as described in Material and Methods. (B) Specific gene amplicon plots in PC-3 control and quercetin-treated cells for β-actin, p53, and CDK2 genes. Rn, normalized fluorescence; TAI, transcript accumulation index.
Hari Krishnan Nair et al. Clin. Diagn. Lab. Immunol. 2004; doi: /CDLI