1. Therapeutic administration of Areg myogenesis during infection.
Therapeutic administration of Areg myogenesis during infection. (A) Whole-muscle mRNA expression of muscle repair–associated genes pax7, pax3, igf1, myf5, myod, and myog normalized to gapdh relative to PBS-treated skeletal muscle. (B) WGA (green) and DAPI (blue) staining of skeletal muscle sections. Scale bar, 500 μm. (C) Average (top) and frequency distribution (bottom) of myofiber cross-sectional area. Error bars represent maximum and minimum. (D) Percentage of damaged area relative to total tissue area determined by percentage of occupied space of binarized WGA staining. Only the TA tissue was analyzed. Peripheral tissue and connective tissue debris were excluded from analysis [* in (B)]. Results are representative of three experiments of n ≥ 4 mice per group per experiment; error bars represent SD. ***p < 0.001, ****p < , Student t test (C [top]), Mann–Whitney U test (D), Kolmogorov–Smirnov test (C [bottom]).
Richard M. Jin et al. ImmunoHorizons 2018;2:
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