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Figure 1 GABAB expression in the thymus(A–C) Staining of thymus tissue with anti-cytokeratin (A) and anti-GABAB antibody (B, C double immunofluorescence).

Published byEmilie Bjerre Modified over 5 years ago

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Presentation on theme: "Figure 1 GABAB expression in the thymus(A–C) Staining of thymus tissue with anti-cytokeratin (A) and anti-GABAB antibody (B, C double immunofluorescence)."— Presentation transcript:

1 Figure 1 GABAB expression in the thymus(A–C) Staining of thymus tissue with anti-cytokeratin (A) and anti-GABAB antibody (B, C double immunofluorescence). GABAB expression in the thymus(A–C) Staining of thymus tissue with anti-cytokeratin (A) and anti-GABAB antibody (B, C double immunofluorescence). Cytokeratin-positive epithelial cells express GABAB receptor (arrows). In addition, GABAB is expressed in other cell types of the thymus, most likely T cells (arrowheads). (D–F) Staining of thymus tissue with anti-cytokeratin (D) and patient sera (E, F double immunofluorescence). Patient antibodies clearly co-localize with cytokeratin-positive epithelial cells. The pattern of staining and co-localization in these areas is identical to panels A, B, and C. Due to antibody combination and tissue availability restrictions, we were unable to triple stain for cytokeratin-GABAB-serum. From our results it can be safely inferred that the GABAB serum autoantibodies bind GABAB that is expressed in thymic epithelial cells. GABAB = γ-aminobutyric acid receptor B. Harry Alexopoulos et al. Neurol Neuroimmunol Neuroinflamm 2014;1:e39 © 2014 American Academy of Neurology

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