1. Transfer of energy via ATP

2. Higher Biology (January 2011)
UNIT 2: Metabolism and Survival
1. Metabolism is essential for Life
c) Cellular respiration
(i) Transfer of energy via ATP
Experiments on ATP dependent reactions, e.g. luciferase, luminescent reactions

3. Higher Human Biology (January 2011)
UNIT 1: Human Cells
3. Cell metabolism
b) Cellular respiration
(i) Transfer of energy via ATP
Experiments on ATP dependent reactions, e.g. luciferase, luminescent reactions

5. Luminescence
Luminescence is emission of light by a substance not resulting from heat; it is thus a form of cold body radiation
Source:
(accessed June 22nd 2012)

6. Bioluminescence

8. George McGavin
Edith Widder

20. Flashlight fish
Angler fish

23. Bioluminescence -
Lifesaver?

26. Firefly bioluminescence:
Emission oxidation of luciferin
Proteins and substrates - mostly unrelated
Most referred to as luciferin / luciferase.

27. Activity
Two practicals:
Cold
Heat
Adapt to the dark
10 min (approx) for each practical

28. Equipment / materials
2 Petri dishes
Disposable pipettes
Bijou / small bottle of ATP
Bijou of buffer (on ice)
Test-tube
Water 60oC
1 (and only 1!) small bottle of firefly tails
Red light