1. Predicted location and functional classification of differentially expressed transcripts.
Predicted location and functional classification of differentially expressed transcripts. Transcripts were classified according to the subcellular location and predicted function of their encoded protein product, using the UniProt database. (A) Cellular location analysis shows that 16/37 transcripts were predicted to encode cytoplasmic proteins. (B) The function of 10 out of 37 transcript gene products could not be classified, either because they were absent from the UniProt database or because their function was unknown. Six transcripts encoded proteins predicted to be involved in metabolism, highlighting possible metabolic differences between disease- and carriage-associated isolates. (C) Venn diagram listing differentially expressed (log2Δ ≥ ±1; adjusted P < 0.05) transcripts between each carriage isolate and the disease isolate (NZ97/192). Five transcripts, highlighted in red, were differentially expressed in all three carriage isolates, relative to the invasive isolate. For detailed transcript classification, see Table S2.
Xiaoyun Ren et al. mSystems 2017; doi: /mSystems