1. Analysis of total and polysomal RNA clk-1(qm30) and clk-1(qm30)+WT.
Analysis of total and polysomal RNA clk-1(qm30) and clk-1(qm30)+WT. (A) Schematic representation of polysomal fraction (blue) used for RNA isolation and total RNA (green) from C. elegans mutants clk-1(qm30) and clk-1(qm30)+WT that were used for RNAseq. (B) Fold changes of differentially expressed genes in the total RNA of clk-1(qm30) versus clk-1(qm30)+WT plotted against fold changes in polysomal RNA of clk-1(qm30) versus clk-1(qm30)+WT. Colors of the individual data points correspond to the colors of the groups of genes in the Venn diagram in (C). (C) Venn diagram showing 1657 genes were similarly up-regulated and 2574 genes down-regulated in both the polysomal as the total RNA in the clk-1(qm30) strain compared with clk-1(qm30)+WT. A total of 434 genes were exclusively up-regulated in the polysomal RNA (blue), whereas 2767 genes were exclusively up-regulated in the total RNA (green). Furthermore, 477 genes were exclusively down-regulated in the polysomal RNA (red) and 2628 genes were exclusively down-regulated in the total RNA. Differential expressions (B, C) are with a threshold-adjusted P value < (D) Significant Cluster GO Enrichments (threshold Enrichment Score > 3) associated with the genes specifically up and down-regulated in groups of genes in (C) (colors of the bars correspond again to the color of the groups in (C)). (E) Fib-1 is reduced in both the total and polysomal RNA of clk-1(qm30) compared with clk-1(qm30)+WT. (F, G) FIB-1 levels are reduced in clk-1(qm30) worms compared with N2 worms. Error bars represent mean ± SD, significance was tested with t test, ***P <
Marte Molenaars et al. LSA 2018;1:e
© 2018 Molenaars et al.