Presentation is loading. Please wait.

Presentation is loading. Please wait.

Increased surface expression of NOTCH on memory T cells in peripheral blood from patients with asthma  Irma Tindemans, PhD, Heleen Vroman, PhD, Melanie.

Published byBernadette Butler Modified over 6 years ago

Similar presentations

Presentation on theme: "Increased surface expression of NOTCH on memory T cells in peripheral blood from patients with asthma  Irma Tindemans, PhD, Heleen Vroman, PhD, Melanie."— Presentation transcript:

1 Increased surface expression of NOTCH on memory T cells in peripheral blood from patients with asthma  Irma Tindemans, PhD, Heleen Vroman, PhD, Melanie Lukkes, BSc, Menno van Nimwegen, BSc, Marjolein J.W. de Bruijn, BSc, Bobby W.S. Li, PhD, Alex Kleinjan, PhD, Geertje M. de Boer, MD, Gerdien A. Tramper-Stranders, MD, PhD, Mirjam Kool, PhD, Gert-Jan Braunstahl, MD, PhD, Rudi W. Hendriks, PhD  Journal of Allergy and Clinical Immunology  Volume 143, Issue 2, Pages e3 (February 2019) DOI: /j.jaci Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Proportions of NOTCH1+ and NOTCH2+ cells are increased in various T-cell compartments in peripheral blood from patients with asthma. A-B, Flow cytometric gating of CD3+CD4+ TH cells (excluded for CD127−CD25hi regulatory cells), CD45RA− memory T cells, and CD45RA−CXCR5−CCR6−CCR4+CXCR3− TH2 cells (Fig 1, A). B, An example of NOTCH1 and NOTCH2 expression on T cells. C, Proportions of NOTCH1+/NOTCH2+ CD4− T cells, CD4+ T cells, naive T cells (CD45RA+), memory T cells (CD45RA−), and TH2 cells (CD45RA−CXCR5−CCR6−CCR4+CXCR3−). D, Correlations of proportions of NOTCH1+/NOTCH2+ cells within naive T cells, memory T cells, and TH2 cells, measured in peripheral blood from patients with asthma. E, ACQ scores of patients with high (above median) or low (below median) proportions of NOTCH1+/NOTCH2+ TH2 cells. F, Mean fluorescent intensity of CD25 and CRTH2 on NOTCH1+ and NOTCH1− memory CD4+ T cells. Data were acquired with an LSR II flow cytometer operated using FACS Diva software 6.1 (BD Biosciences, Erembodegem, Belgium) and analyzed using FlowJo (Tree Star Inc, Ashland, Ore). Data are shown as symbols for individual patients/controls, together with horizontal bars for median values. Comparisons between groups (Fig 1, C, E, F) were evaluated using a Mann-Whitney U test. P values were 2-sided and analyses were performed using GraphPad Prism software (version 5.01). P < .05 was considered statistically significant. CRTH2, Chemoattractant receptor-homologous molecule expressed on TH2 cells; FSC-A, forward scatter-area. *P < .05, **P < .01, ***P < .001. Correlations (Fig 1, D) were analyzed using a Pearson correlation test. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Increased surface expression of NOTCH on memory T cells in peripheral blood from patients with asthma  Irma Tindemans, PhD, Heleen Vroman, PhD, Melanie."

Similar presentations

Defective B-cell memory in patients with Down syndrome Ruud H.J. Verstegen, MD, Gertjan J. Driessen, MD, Sophinus J.W. Bartol, BSc, Carel J.M. van Noesel,

Defective B-cell memory in patients with Down syndrome Ruud H.J. Verstegen, MD, Gertjan J. Driessen, MD, Sophinus J.W. Bartol, BSc, Carel J.M. van Noesel,
Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy  Erik Wambre, PhD, Jonathan.

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy  Erik Wambre, PhD, Jonathan.
Specific immunotherapy modifies allergen-specific CD4+ T-cell responses in an epitope- dependent manner  Erik Wambre, PhD, Jonathan H. DeLong, BSc, Eddie.

Specific immunotherapy modifies allergen-specific CD4+ T-cell responses in an epitope- dependent manner  Erik Wambre, PhD, Jonathan H. DeLong, BSc, Eddie.
Increased numbers of activated group 2 innate lymphoid cells in the airways of patients with severe asthma and persistent airway eosinophilia  Steven.

Increased numbers of activated group 2 innate lymphoid cells in the airways of patients with severe asthma and persistent airway eosinophilia  Steven.
The loss of IgM memory B cells correlates with clinical disease in common variable immunodeficiency  Rita Carsetti, MD, Maria Manuela Rosado, PhD, Simona.

The loss of IgM memory B cells correlates with clinical disease in common variable immunodeficiency  Rita Carsetti, MD, Maria Manuela Rosado, PhD, Simona.
Defective B-cell proliferation and maintenance of long-term memory in patients with chronic granulomatous disease  Nicola Cotugno, MD, Andrea Finocchi,

Defective B-cell proliferation and maintenance of long-term memory in patients with chronic granulomatous disease  Nicola Cotugno, MD, Andrea Finocchi,
Defective calcium signaling and disrupted CD20–B-cell receptor dissociation in patients with common variable immunodeficiency disorders  Annick A.J.M.

Defective calcium signaling and disrupted CD20–B-cell receptor dissociation in patients with common variable immunodeficiency disorders  Annick A.J.M.
Defective B-cell proliferation and maintenance of long-term memory in patients with chronic granulomatous disease  Nicola Cotugno, MD, Andrea Finocchi,

Defective B-cell proliferation and maintenance of long-term memory in patients with chronic granulomatous disease  Nicola Cotugno, MD, Andrea Finocchi,
Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling.

Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling.
Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 
Jay A. Lieberman, MD, Faith R. Huang, MD, Hugh A

Jay A. Lieberman, MD, Faith R. Huang, MD, Hugh A
Immunologic defects in 22q11.2 deletion syndrome

Immunologic defects in 22q11.2 deletion syndrome
Probiotic supplementation has little effect on peripheral blood regulatory T cells  Nicholas P. West, PhD, Peggy L. Horn, PhD, David B. Pyne, PhD, Hilary.

Probiotic supplementation has little effect on peripheral blood regulatory T cells  Nicholas P. West, PhD, Peggy L. Horn, PhD, David B. Pyne, PhD, Hilary.
Magdalena A. Berkowska, PhD, Jorn J

Magdalena A. Berkowska, PhD, Jorn J
Type 2 innate lymphoid cells in induced sputum from children with severe asthma  Prasad Nagakumar, MBBS, Laura Denney, PhD, Louise Fleming, MD, Andrew.

Type 2 innate lymphoid cells in induced sputum from children with severe asthma  Prasad Nagakumar, MBBS, Laura Denney, PhD, Louise Fleming, MD, Andrew.
Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells  Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.

Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells  Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.
Dorothy M. Ryan, MD, Stephen J. Fowler, MD, Robert M. Niven, MD 

Dorothy M. Ryan, MD, Stephen J. Fowler, MD, Robert M. Niven, MD 
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?
Notch signaling in T cells is essential for allergic airway inflammation, but expression of the Notch ligands Jagged 1 and Jagged 2 on dendritic cells.

Notch signaling in T cells is essential for allergic airway inflammation, but expression of the Notch ligands Jagged 1 and Jagged 2 on dendritic cells.
Flow cytometric measurement of STAT1 and STAT3 phosphorylation in CD4+ and CD8+ T cells—clinical applications in primary immunodeficiency diagnostics 

Flow cytometric measurement of STAT1 and STAT3 phosphorylation in CD4+ and CD8+ T cells—clinical applications in primary immunodeficiency diagnostics 

Similar presentations

Ads by Google