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The expression of estrogen receptors as well as GREB1, c-MYC, and cyclin D1, estrogen-regulated genes implicated in proliferation, is increased in peritoneal endometriosis Chiara Pellegrini, M.Sc., Ilaria Gori, Ph.D., Chahin Achtari, M.D., Daniela Hornung, M.D., Ph.D., Eric Chardonnens, M.D., Dorothea Wunder, M.D., Maryse Fiche, M.D., Geraldine O. Canny, Ph.D. Fertility and Sterility Volume 98, Issue 5, Pages (November 2012) DOI: /j.fertnstert Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions
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Figure 1 Expression of ERα and ERβ is increased in both ectopic endometrium and peritoneal tissue of patients with endometriosis in comparison with normal subjects. Relative expression of ERα (A) and ERβ (B) genes in normal, eutopic, and ectopic endometrium. mRNA quantification of ERα (C) and ERβ (D) in uninvolved peritoneal tissue from control individuals and patients with endometriosis. *P<.05, **P<.001, vs. normal tissue; νP<.05, ννP<.0001, vs. eutopic endometrium. Data represent mean ± SEM. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions
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Figure 2 Augmented expression of estrogen-regulated genes implicated in proliferation in ectopic endometrial tissue. Expression of E2-regulated genes was analyzed by qRT-PCR and normalized to GAPDH. c-myc (A), cyclin D1 (B), and GREB1 (C). *P<.05, **P<.001, ***P<.0001 vs. normal endometrium; νP<.05 vs. eutopic endometrium. Data are presented as mean ± SEM. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions
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Figure 3 Immunohistochemical localization of estrogen-regulated genes in normal, eutopic, and ectopic endometrium. c-myc expression in normal endometrium (A), eutopic (B), and ectopic endometrial tissue from subjects not taking contraceptives (C). Cyclin D1 expression in normal endometrium (D), eutopic (E), and ectopic endometrial tissue (F). GREB1 expression in normal endometrium (G), eutopic (H), and ectopic (I) endometrial tissue from subjects not taking contraceptives. A picture at ×40 magnification is shown in the insert. Negative control for normal endometrium (J), eutopic (K), and ectopic endometrial tissue (L). A representative of six biological replicates is shown. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions
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