1. Ch. 13 Genetic Engineering
Unit 4 Genetics
Ch. 13 Genetic Engineering

2. Selective Breeding
Selective breeding - allowing animals with desired characteristics to reproduce
It takes advantage of naturally occurring genetic variation in plants, animals, etc.

3. Selective Breeding
Humans use selective breeding to pass desired traits on to the next generation of organisms
Nearly all domestic animals (horses, cats, etc.) & most crop plants have been produced by selective breeding

4.  Selective Breeding
Hybridization - crossing dissimilar individuals to bring together the best of both organisms
The organisms produced, hybrids, are usually hardier than both parents

5. Selective Breeding
Inbreeding - continued breeding of individuals with similar characteristics
Many breeds of dogs are maintained by inbreeding
It helps to ensure the characteristics of each breed are preserved

6. Problems with Selective Breeding (Inbreeding)
Larger chance of inheriting genetic diseases
Lower life expectancy
Lack of genetic variation

7. Increasing Variation
Alternatively, selective breeding can increase genetic variation in a population by causing mutations to occur
Selective breeding was used to create oil-eating bacteria, used to clean up oil spills

8. Manipulating DNA Scientists study & change DNA molecules
Different techniques are used to extract (remove) DNA from cells, to cut DNA into smaller pieces, to identify the sequence of bases in a DNA molecule, & to make unlimited copies of DNA

9. The Tools of Molecular Biology
Genetic engineering - making changes in the DNA code of a living organism

10. The Tools of Molecular Biology
Cutting DNA:
DNA molecules are too large to be analyzed, so biologists cut them precisely into smaller fragments
Restriction enzymes enzymes that cut DNA at a specific sequence of nucleotides

11. The Tools of Molecular Biology
Separating DNA:
Gel electrophoresis - a mixture of DNA fragments is placed at 1 end of a gel, & an electric voltage is applied to the gel
When the power is turned on, DNA fragments move toward the + end of the gel
Smaller DNA fragments move faster & farther

12. Gel Electrophoresis

13. Using Gel Electrophoresis
Gel electrophoresis can be used for paternity tests and to solve crimes

14. Gel Electrophoresis- Paternity Testing

15. Using the DNA Sequence Cutting & pasting: DNA sequences can be changed
Restriction enzymes - used to take a gene from 1 organism & attach it to the DNA of another organism
Recombinant DNA - DNA molecules produced by combining DNA from different sources

16. CRISPR
CRISPR allows genetic material to be added, removed, or altered at particular locations in the genome

17. Potential Uses of CRISPR
Curing disease
Preventative medicine
Assignment- Look up the word CRISPR in the news and give a summary of the news article.

18. Using the DNA Sequence Making copies:
Polymerase chain reaction (PCR) - technique that allows biologists to make many copies of a particular gene

19. Cell Transformation
During transformation, a cell takes in DNA from the outside (DNA from another organism)
That external DNA combines with the cell’s DNA
As the cell copies its own DNA, it also copies the external DNA, since the 2 were combined during transformation

20. Transforming Bacteria
Plasmid - circular DNA of bacteria

21. Applications of Genetic Engineering
Genetic engineering makes it possible to transfer DNA sequences, including whole genes, from 1 organism to another
It has spurred the growth of biotechnology, a new industry that is changing the way we interact with the living world

22. Transgenic Organisms
Transgenic - organisms have genes from other species
A gene from 1 organism can be inserted into cells from another organism
Those transformed cells can be used to grow new organisms

23. Transgenic Organisms Transgenic Animals:
They have been used to study genes & improve the food supply (also known as GMO- Genetically Modified Organisms)
Mice have been produced with human genes that make their immune systems similar to ours
Livestock have extra copies of human growth hormone

24. Transgenic Organisms Transgenic Plants:
Many crop plants contain genes that produce a natural insecticide
Others have genes that allow them to resist weed-killing chemicals
Rice has been genetically modified to contain Vitamin A, essential for human health

25. What are some pros and cons of GMOs?

26. Cloning
Clone - a member of a population of genetically identical cells produced from a single cell

27. Cloning in the (Recent) News

28. Cloning