1. Practical Biochemistry 2 Lab BIOCB2101
IUG, Fall 2017
Dr Tarek Zaida

2. Office hours:
Sat, Mon, Wed: 11-12
Sun: 11-12:30

3. Course Description
This course is the second part of a continuous series of experiments designated for the students whose major is Biochemistry, with the aim to improve their ability in dealing with the soul of biochemistry through some selected experiments.

4. Course Objective
By the end of this course students will be:
Ready to explain the different roles of the very important biomolecules in the living system, through understanding the biochemical properties of such compounds.
More self-confident in conducting experiments with their own hands.
Able to command and handle the different lab equipments with confidence.
Familiar with the different methods applied in biochemistry lab. 
Students will:
gain communication skills
Will get used to team work

5. Experiments Lab 1: Active Acidity, pH, and Buffers
Lab 2: Determination of Reducing- and Non-
reducing Sugars
Lab 3: Determination of Ascorbic Acid
Iodometrically
Lab 4: Paper Chromatography of Sugars and
Amino acids
Lab 5: Separation of blood serum lipids by
thin-layer chromatography

6. Lab 6: Quantitative Determination of Protein
in Foods by the Biuret Method
Lab 7: Quantitative Determination of Protein
by the Bradford method
Lab 8: Analysis of pigments and oxidation
processes in photosynthetic organisms.
Lab 9:Energy Metabolism.
Lab 10: Isolation of Cholesterol from Egg Yolk
Lab 11& 12: Isolation & Purification of LDH from animal tissues.

7. Textbook
PPT presentations of Dr. Tarek Zaida

8. Evaluation
There will be different types of evaluation that will include:
Quizzes
Writing Lab reports
Participation in the actual lab work.
Participation in the discussion with classmates through the electronic learning tools.

9. Course Policy Attendance all lab sessions is a mandatory.
Zero tolerance for not following given instructions during lab sessions.
Wearing the lab coat as soon as you step in the lab.
Acting responsibly during the entire lab session.
Team work is a must, including communication with group partners.
Giving up reports from the previous lab session must be on the day of the next lab only.
Quizzes will be given only electronically on a given previously announced date and time.
No make ups are allowed.

10. After each Lab activity
Quizzes 12
On demand
Reports 36
After each Lab activity
Active Engagement during lab session 20
Throughout the term
Pre-lab Quizzes
At the end of term
Practical exam
Total grades
100