1. Biochemistry II BCM311 By: Zatilfarihiah Rasdi

2. Objectives  Upon completion of this course, students should be able to:  Define the basic terms and concepts in enzyme kinetics, metabolism and electron transport system  Describe the functions, reactions and control of selected meatbolic pathways of carbohydrates, amino acids, lipids metabolisms  Show the production and consumption of “high energy” compounds like ATP, production and consumption of reducing power in the pathways studied

3. Credit hours: 2 Lecture : 2hrs/week Monday : 2.10 – 4.00 pm Venue: BS5 Lecture : 2hrs/week Wednesday: 2.10 – 4.00 pm Venue: BS7 Assessment Tests- 40% Final exam.- 60%

4. Lesson plan

5. Lab class  Colorimetric tests for carbohydrates  Chromatography  Saponification value  Acid value  Qualitative tests for amino acid  Tests for proteins  The pH meter  Paper chromatography  Assay for proteins, reducing sugar  Determination of KM and Vmax  Electrophoresis and chromatography practice in BCM312  Campbell, M.K., and Farrell, S.O. (2009) Biochemistry, 6 th ed. Thompson Brooks/Cole  Boyer, R. (2005) Concepts in Biochemistry, 3 rd ed. Wiley Additional text  Voet, D.J, Voet, G.V, and Pratt, C.W (2008) Principle of Biochemistry, 3 rd ed. Wiley Reference