1. AS/NZS 4187:2014 Water Quality Analysis
Zachary Chataway

2. 4187 Guideline Presentation- March 2015
Topics
AS/NZS 4817:2014
Water Quality Parameters
What is TVC?
What are Endotoxins?
Sampling & Transport
Guideline Report
4187 Guideline Presentation- March 2015

3. 4187 Guideline Presentation- March 2015
1. AS/NZS 4817:2014
AS/NZS 4817: Reprocessing of reusable medical devices in health service organizations.
Replaces AS/NZS 4817: Cleaning, disinfecting and sterilizing reusable medical and surgical instruments and equipment, and maintenance of associated environments in health care facilities.
4187 Guideline Presentation- March 2015

4. 2. Water Quality Parameters
Page 55 of AS/NZS 4187:2014
4187 Guideline Presentation- March 2015

5. 4187 Guideline Presentation- March 2015
3. What is TVC?
TVC = Total Viable Count
HCC, HPC, TCC
Indication of the size of the microbial community in a water system
Microbes will flourish with no disinfection measure present ie Chlorine
4187 Guideline Presentation- March 2015

6. 3. What is TVC – how is it measured?
Pour plate – 1ml inoculation – growth medium added and incubated at a known temperature for 2-3 days. Potable =22 and 36 deg C respectively
Membrane Filtration (MF) – 100ml inoculation - growth medium added and incubated at a temperature between 30 – 35 deg C for 5 days
4187 Guideline Presentation- March 2015

7. 3. What is TVC – how is it measured?
4187 Guideline Presentation- March 2015

8. 3. What is TVC – how is it measured?
4187 Guideline Presentation- March 2015

9. 4. What are Endotoxins & How are they analysed?
Endotoxins are large molecules consisting of a lipid and a polysaccharide (LPS) and are found in the outer membrane of Gram-negative bacteria
Endotoxins are often released upon cell death but can be excreted by gram –ve bacteria as a defence mechanism
Endotoxins when introduced into human body, can cause a fever-like reaction
4187 Guideline Presentation- March 2015

10. 4. What are Endotoxins & How are they analysed?
ALS currently perform Bacterial Endotoxin analysis in our Brisbane laboratory
Based on clotting reaction of horseshoe crab blood
Sample is incubated with an LAL standard (Limulus Amebocyte Lysate) at a known sensitivity and the clotting time is measured and plotted on a standard curve
The sample is collected in a sterile & non-pyrogenic 50mL tube
HT of 24hrs – currently running extended HT data
TAT 5-7 days – Fast TAT available (1 day)

11. 5. Sampling & Transport to the Laboratory
Aseptically collected – flaming / ethanol
Appropriate containers – sterile, non-pyrogenic
Correct chilling - <4 deg on receipt
Holding time – TVC analysed
within 24hrs of collection

12. 6. Guideline Report

13. 4187 Guideline Presentation- March 2015
7. Questions
4187 Guideline Presentation- March 2015