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Monica G. Lawrence, MD, Jennifer W. Leiding, MD, Jonathan J

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Presentation on theme: "Monica G. Lawrence, MD, Jennifer W. Leiding, MD, Jonathan J"— Presentation transcript:

1 GATA3 haploinsufficiency does not block allergic sensitization or atopic disease 
Monica G. Lawrence, MD, Jennifer W. Leiding, MD, Jonathan J. Lyons, MD, Amy P. Hsu, BS, Celeste C. Nelson, CRNP, Nina Jones, RN, Alan Fitzgerald, RN, Wade W. Chien, MD, Lisa Workman, BA, Thomas A. Platts-Mills, MD, Carmen Brewer, PhD, Rachel I. Gafni, MD, Kelly D. Stone, MD, PhD, Joshua D. Milner, MD, Steven M. Holland, MD  Journal of Allergy and Clinical Immunology  Volume 137, Issue 2, Pages e2 (February 2016) DOI: /j.jaci Copyright © Terms and Conditions

2 Fig 1 Results of a 6-hour stimulation of PBMCs with PMA and ionomycin in the presence of brefeldin A, followed by fixation, permeabilization, and staining for intracellular cytokines. Data shown represent the mean of 3 independent experiments for GATA3+/− patients; included for comparison are age- and sex-matched healthy controls. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © Terms and Conditions

3 Fig 2 CD4+ naive T cells were subject to TH0 (anti-CD3/CD28 and IL-2) or TH2 (anti-CD3/CD28, IL-2, IL-4, anti–IFN-γ, anti–IL-10)-skewing conditions before staining for expression of T-bet and GATA3. n = 3-5 independent experiments. A, Geometric mean fluorescent intensity (MFI) of GATA3 under TH0 and TH2 conditions (representative data). B, Fold increase of GATA3 MFI under TH2 conditions compared with TH0 conditions. PMA, Phorbol 12-myristate 13-acetate. Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci ) Copyright © Terms and Conditions


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