1. A Controlled Study of Colonic Immune Activity and β7+ Blood T Lymphocytes in Patients With Irritable Bowel Syndrome 
Lena Öhman, Stefan Isaksson, Anna Lundgren, Magnus Simrén, Henrik Sjövall 
Clinical Gastroenterology and Hepatology 
Volume 3, Issue 10, Pages (October 2005)
DOI: /S (05)
Copyright © 2005 American Gastroenterological Association Terms and Conditions

2. Figure 1
The frequency of integrin β7+ blood T cells and colonic expression of MAdCAM-1. (A) The expression of integrin β7 on CD4+ and CD8+ T cells isolated from peripheral blood of patients with IBS, UC, and controls was analyzed by flow cytometry. Each symbol represents the frequency of β7 positive cells detected among the CD4+ and CD8+ cell population, respectively, isolated from one individual. Median values are indicated by horizontal bars. (n): IBS = 18, UCr = 5, UCa = 7, CTRL = 8. (B) Serial sections of biopsies from the ascending and the sigmoid colon, respectively, were stained for endothelial cells (factor VIII) or MAdCAM-1. Each symbol represents the frequency of MAdCAM-1 positive endothelial tissue (VIII+) detected from one individual. Median values are indicated by horizontal bars. (n): IBS = 11/9, UCr = 4/4, UCa = 4/4, CTRL = 8/8 in the ascending and the sigmoid colon, respectively. (C) Biopsies were cultured in the presence of 5 μg/mL phytohemagglutinin for 24 hours, and the secretion of IFN-γ was measured by specific ELISA. Each symbol represents the IFN-γ secretion per mg tissue, detected from one individual. (n): IBS = 11/11, UCr = 6/6, CTRL = 4/4 in the ascending and the sigmoid colon, respectively. Median values are indicated by horizontal bars.
Clinical Gastroenterology and Hepatology 2005 3, DOI: ( /S (05) )
Copyright © 2005 American Gastroenterological Association Terms and Conditions

3. Figure 2
The frequency of integrin β7+ T cells in the colonic mucosa. The expression of integrin β7 on CD4+ and CD8+ T cells isolated from the ascending and the sigmoid colon LP was analyzed by flow cytometry. (n): IBS = 14/15, UCr = 6/6, UCa = 6/6, CTRL = 7/6 in the ascending and the sigmoid colon, respectively. Each symbol represents the frequency of β7 positive cells detected among the CD4+ and CD8+ cell population, respectively, isolated from one individual. Median values are indicated by horizontal bars. LPL A, LP lymphocytes from ascending colon; LPL S, LP lymphocytes from sigmoid colon.
Clinical Gastroenterology and Hepatology 2005 3, DOI: ( /S (05) )
Copyright © 2005 American Gastroenterological Association Terms and Conditions

4. Figure 3
The frequency of naïve CD45RA+ T cells in the colonic mucosa. The expression of CD45RA on CD4+ and CD8+ T cells isolated from the ascending and the sigmoid LP was analyzed by flow cytometry. (n): IBS = 15/16, UCr = 4/5, UCa = 6/6, CTRL = 7/5 in the ascending and the sigmoid colon, respectively. Each symbol represents the frequency of positive cells detected among the CD4+ and CD8+ cell population, respectively, isolated from one individual. Median values are indicated by horizontal bars. LPL A, LP lymphocytes from ascending colon; LPL S, LP lymphocytes from sigmoid colon.
Clinical Gastroenterology and Hepatology 2005 3, DOI: ( /S (05) )
Copyright © 2005 American Gastroenterological Association Terms and Conditions