1. Chapter 12
Structure of Nucleic Acids

2. 12.1 The Primary Structure of Nucleic Acids

3. The chain termination or dideoxy method of DNA sequencing

4. Automated DNA Sequencing

5. 12.2 Secondary Structure of DNA
DNA double helix
right-handed
10bp per turn
0.34nm
d = 2nm

6. Base stacking force

7. antiparallel

9. 12.3 Denaturation and Renaturation of DNA
DNA Denaturation

10. DNA Renaturation

12. 12.3.3 Nucleic Acid Hybridization
Buoyant Density of DNA

13. Density gradient centrifugation

14. 12.4 Supercoil and Cruciform : Tertiary structure in DNA

15. Relaxed DNA
Superhelix DNA
Unwound DNA

16. cruciform
Palindromic sequence

17. 12.5 Secondary and Tertiary Structure of RNA
Unusual bases of RNA

18. Messenger RNA ( mRNA )
5`-cap `-poly(A)
7-methyl-G-cap

19. Transfer RNA ( tRNA )
Cloverleaf secondary structure of tRNA

22. Tertiary interactions in yeast phenylalanine tRNA

23. L-shaped tertiary form

24. Ribosomal RNA ( rRNA )

25. The proposed secondary structure for E.coli 16S rRNA