1. Melting behavior of proteins identified in the Escherichia coli meltome and their properties
Melting behavior of proteins identified in the Escherichia coli meltome and their properties AReproducibility of identified proteins in each replicate of E. coli meltome analysis.BOverlap of identified proteins with previously published proteomics datasets obtained from E. coli.CDistribution of differences between protein abundance after being extracted with NP‐40 or with SDS.D, ECorrelation of melting point with (D) protein abundance (r = 0.06, P = 0.015, as measured by the top3 intensity corresponding to the lowest temperature) and (E) molecular weight (r = −0.08, P = 0.0009).FCorrelation of melting point in living cells with melting point in lysate—both from TPP (r = 0.82, P < 0.0001).GMelting curves for E. coli outer membrane proteins. The average melting curve for each class of outer membrane proteins is shown.HDistribution of melting temperatures (Tm) of the E. coli proteome according to their cellular compartment.IFraction of proteins with Tm > 87°C (the highest temperature tested) in each cellular compartment.
André Mateus et al. Mol Syst Biol 2018;14:e8242
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