1. NSAF and GeneChip data have similar distribution properties.
NSAF and GeneChip data have similar distribution properties. A-C, the rowMean value was calculated for each transcript or protein in the indicated dataset and subsequently transformed to its base 10 logarithm. The black line in each plot represents the density distribution of all log10(rowMean) values in the corresponding dataset. The blue lines in each plot represent the density distribution of log10(rowMean) values of transcripts or proteins that were detected with a specific number of absent calls (in the case of the GeneChip dataset) or zero values (in the case of the NSAF datasets). The color intensity of the blue lines was chosen from a gradual color palette to reflect the actual number of absent or zero values according to the color bar depicted at the bottom of the figure. D–F, the rowS.D. value for each transcript or protein was measured across all available replicates in the indicated dataset and subsequently transformed to its base 10 logarithm. The density distributions of the log10(rowS.D.) values were plotted according to the same color coding scheme described for the upper panels. A and D represent mouse GeneChip data, B and E represent yeast NSAF data, and C and F represent Mediator NSAF data.
Norman Pavelka et al. Mol Cell Proteomics 2008;7:
© 2008 The American Society for Biochemistry and Molecular Biology