1. Validation of the CCE associations by mass spectrometry.
Validation of the CCE associations by mass spectrometry. (A) Between 49 and 59% of the 7000 CCE associations correspond to true physical associations, as estimated with shotgun proteomics elution profiles. The extent of co‐elution of positive control (literature; Joshi‐Tope et al, 2005), negative control (random), and CCE associations were calculated as the Pearson correlation coefficients between interaction partners’ elution profiles, defining a correlation coefficient histogram for each set of associations. The proportion of true positives in the 7000 CCE associations was estimated by fitting the CCE correlation coefficient histogram as a linear mixture of the control histograms, with the true‐positive rate corresponding to the percentage of the positive control histogram providing the best fit (inset). (B) Specific examples of correlated elution profiles for CCE partners, supporting the physical association of these protein pairs.
Arun K Ramani et al. Mol Syst Biol 2008;4:180
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