1. Agenda 4/24 Recombinant DNA warm up Gel Electrophoresis Techniques
Biotech Review Questions
Homework
PCR Video and Notes

2. Recombinant DNA Steps
From memory, attempt to write out the steps of recombinant DNA using a specific example

3. Solve the crime…

4. Polymerase Chain Reaction (PCR)
When a DNA sample is found, it must be larger enough to work with.
PCR is used to amplify the sample of DNA into a big enough sample to analysis using gel electrophoresis

5. PCR

6. Restriction Enzymes
Restriction enzymes cut at specific sequence in DNA
They are used in gel electrophoresis to create unique ‘cuts’ for each person
These smaller pieces of DNA are then loaded into a gel to be analyzed
There are also used in recombinant DNA to cut out the gene of interest

7. Restriction Enzymes

8. Gel Electrophoresis Overview

9. Gel Electrophoresis Smaller pieces of DNA travel farther
A ‘comparison’ DNA sample is used
Can be used to determine size
Or to match an unknown sample
DNA travels from the negative side (anode) to the positive side (cathode)
What does that mean about the charge of DNA?

10. Gel Electrophoresis