Presentation is loading. Please wait.

Presentation is loading. Please wait.

Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,

Published byPetra Kis Modified over 5 years ago

Similar presentations

Presentation on theme: "Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,"— Presentation transcript:

1 Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library 
Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr, MD, Isabella Schöll, MSc, Regina Knittelfelder, Sabine Flicker, PhD, Susanne Spitzauer, MD, Rudolf Valenta, MD, George Boltz-Nitulescu, PhD, Otto Scheiner, PhD, Erika Jensen-Jarolim, MD  Journal of Allergy and Clinical Immunology  Volume 114, Issue 6, Pages (December 2004) DOI: /j.jaci Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 A, Phylogenetic tree indicating the degree of similarity between the selected peptides. Identical residues are boxed. B, Alignment of peptides with the amino acid sequences of Phl p 5a and Phl p 6. Identical residues are indicated in bold, and physicochemically related amino acids are indicated in bold-faced italics. Additionally, identical and related amino acids are boxed in gray. Predicted α-helices are underlined. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 Molecular modeling and IgE epitope mapping on the 2 AR repeats of Phl p 5a. A and B, Ribbon diagrams visualizing the AR repeats. C and D, Molecular surface representations. Alignment regions with the peptides (designations correspond to clone numbers in Table I) are highlighted in red (AR repeat 1) and blue (AR repeat 2), respectively. E and F, One hundred eighty degree rotation of the 2 domains. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 Phage-displayed peptides bind specifically to a monoclonal Phl p 5a–reactive Fab fragment (black columns) and to Phl p 5a IgE (white columns) but not to the isotype controls PS- and SUS11-IgE (gray columns). Twelve individual phage clones (1 × 108 cfu/mL) and WT-phage lacking an insert were added to the coated wells. The data represent the mean ± SD of 4 individual assays. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5 Fig 4 A-C, Phage clones of the acidic (1) and competitive (2) elution method and a phage cocktail (3) tested for IgE specificity in ELISA. D-F, BIAcore sensorgrams of Phl p 5–specific IgE binding to Phl p 5a. G-I, Bet v 1–specific IgE binding to Bet v 1 in the absence of phages (IgE), in the presence of WT-phage, and in the presence of phage clones. One typical sensorgram of 3 individual measurements is shown. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

6 Fig 5 IgG reactivity of Biozzi mice immune sera elicited by phagotopes F1, F29, H32, and WT-phage toward Phl p 5a (A) and Bet v 1 (B). The x-axis shows the time schedule of blood taking. The y-axis shows mean IgG reactivities and SDs of mouse groups (n=5), testing mice individually. ∗∗P < .05 for F1- and F29-phage immune sera on day 35 compared with preimmune sera and WT-phage sera on day 35. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Download ppt "Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,"

Similar presentations

Usefulness of recombinant γ-gliadin 1 for identifying patients with celiac disease and monitoring adherence to a gluten-free diet  Bharani Srinivasan,

Usefulness of recombinant γ-gliadin 1 for identifying patients with celiac disease and monitoring adherence to a gluten-free diet  Bharani Srinivasan,
Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns  Christian S. Hansen, PhD, Martin Dufva,

Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns  Christian S. Hansen, PhD, Martin Dufva,
Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R
Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: Seed storage proteins as common food allergens  Kirsten Beyer,

Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: Seed storage proteins as common food allergens  Kirsten Beyer,
Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,

Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,
Antacid medication inhibits digestion of dietary proteins and causes food allergy  Eva Untersmayr, MD, Isabella Schöll, MSc, Ines Swoboda, PhD, Waltraud.

Antacid medication inhibits digestion of dietary proteins and causes food allergy  Eva Untersmayr, MD, Isabella Schöll, MSc, Ines Swoboda, PhD, Waltraud.
Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.

Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.
Prediction of IgE-binding epitopes by means of allergen surface comparison and correlation to cross-reactivity  Fabio Dall'Antonia, PhD, Anna Gieras,

Prediction of IgE-binding epitopes by means of allergen surface comparison and correlation to cross-reactivity  Fabio Dall'Antonia, PhD, Anna Gieras,
Determination of allergen specificity by heavy chains in grass pollen allergen–specific IgE antibodies  Elisabeth Gadermaier, PhD, Sabine Flicker, PhD,

Determination of allergen specificity by heavy chains in grass pollen allergen–specific IgE antibodies  Elisabeth Gadermaier, PhD, Sabine Flicker, PhD,
Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy  Raphaela.

Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy  Raphaela.
Molecular mimicry between cockroach and helminth glutathione S-transferases promotes cross-reactivity and cross-sensitization  Helton C. Santiago, MD,

Molecular mimicry between cockroach and helminth glutathione S-transferases promotes cross-reactivity and cross-sensitization  Helton C. Santiago, MD,
Plantago lanceolata: An important trigger of summer pollinosis with limited IgE cross- reactivity  Gabriele Gadermaier, PhD, Stephanie Eichhorn, MSc, Eva.

Plantago lanceolata: An important trigger of summer pollinosis with limited IgE cross- reactivity  Gabriele Gadermaier, PhD, Stephanie Eichhorn, MSc, Eva.
Delineating the specificity of an IgE-encoding transcriptome

Delineating the specificity of an IgE-encoding transcriptome
Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,

Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,
Act d 12 and Act d 13: Two novel, masked, relevant allergens in kiwifruit seeds  Sofía Sirvent, PhD, Belén Cantó, BSc, Javier Cuesta-Herranz, MD, PhD,

Act d 12 and Act d 13: Two novel, masked, relevant allergens in kiwifruit seeds  Sofía Sirvent, PhD, Belén Cantó, BSc, Javier Cuesta-Herranz, MD, PhD,
Early recovery from cow's milk allergy is associated with decreasing IgE and increasing IgG4 binding to cow's milk epitopes  Emma M. Savilahti, MD, Ville.

Early recovery from cow's milk allergy is associated with decreasing IgE and increasing IgG4 binding to cow's milk epitopes  Emma M. Savilahti, MD, Ville.
Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy  Julie Wang, MD,

Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy  Julie Wang, MD,
Usefulness of recombinant γ-gliadin 1 for identifying patients with celiac disease and monitoring adherence to a gluten-free diet  Bharani Srinivasan,

Usefulness of recombinant γ-gliadin 1 for identifying patients with celiac disease and monitoring adherence to a gluten-free diet  Bharani Srinivasan,
Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns  Christian S. Hansen, PhD, Martin Dufva,

Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns  Christian S. Hansen, PhD, Martin Dufva,
Incomplete digestion of codfish represents a risk factor for anaphylaxis in patients with allergy  Eva Untersmayr, MD, Helle Vestergaard, Hans-Jørgen.

Incomplete digestion of codfish represents a risk factor for anaphylaxis in patients with allergy  Eva Untersmayr, MD, Helle Vestergaard, Hans-Jørgen.

Similar presentations

Ads by Google