1. MECHE UPDATE 30/03/2011

2. RECRUITMENT MALES FEMALES
Participants (n)
100
114
Caucasian (%) 91 97
Age (yrs)
32.0 ± 9.7
31.4 ± 11.3
BMI (kg.m2)
26.2 ± 4.0
24.4 ± 5.0
Waist (cm)
88 ± 12
79 ± 13
Normal weight (%) 42 70
Overweight (%) 47 23
Obese (%) 11 7
56%
44%

3. PAIRED TEST MEAL PROTOCOLS
MALES
FEMALES
OLTT– OLTT 28 35
OGTT– OLTT 33 38
OGTT– OGTT 63 71 66
200 pairs

4. ADIPOSE TISSUE SAMPLES
MALES
FEMALES
Paired samples 4 5
Single samples 8
No. candidates refusing biopsy 6 4% 6% 5%
15% Cohort eligible for biopsy
10% gave a sample

5. ASSOCIATED INFORMATION
No.
204
Dexa scans, Metabolic rate, Bod pod, Fitness, Strength Analysis underway
205
Self report dietary habits (FFQ)
Analysis complete
204
Self report physical activity (EPAQ)
Analysis to be completed April 2011

6. Metabolic syndrome biochemistry
SAMPLE ANALYSIS
Metabolic syndrome biochemistry
Daytona autoanalyser
Basic biochemical analysis underway
Cholesterol, HDL, TGs, Glu, NEFAs, APOs
Forecasted completion date: Early June

7. Protein profiling & Proteomics
Evidence investigator platform
Metabolic array almost complete
Insulin-Leptin-Resistin-PAI1-Cpeptide
Cytokine array work underway Il’s-VEGF-TNFa-Ifg-MCP1
To be completed Mid April
Adhesion molecules array yet to start ICAM-VCAM-E/P/L-selectin

8. Metabolomics analysis
NMR fasting urine samples
190 postprandial samples
Total 404 urine samples
GCMS - 91 fasting urine samples

9. Transcriptomic analysis
Work on 4 sub-cohorts underway
1 (n=10, lean, 18-30yrs) PBMC gene expression, Single gene and pathway analysis, ELISAs
2 & 3 (n=8, 18-30/>30kg.m2, 30-60yrs) Arrays in progress
4 (n=9, adipose tissue biopsy group)
RNA isolated, Arrays in progress
Results forecasted: Summer

10. Genotyping
. Sample collection complete and all samples (n=212) sent to TCD biobank
Awaiting DNA extraction??
Item 4 : Agenda

11. CLINICAL VARIABLES DATABASE SAMPLE ANALYSIS DATABASE
STATUS OF DATABASES
CLINICAL VARIABLES DATABASE
SAMPLE ANALYSIS DATABASE
SPORTS DATABASE
FFQ DATABASE

12. STATUS OF DATABASES METABOLOMICS PROTEOMICS TRANSCRIPTOMICS CLINICAL
BIOCHEMISTRY
FFQ
METABOLOMICS
SPORTS
PHYSICAL ACTIVITY
PROTEOMICS
TRANSCRIPTOMICS