1. Transthoracic needle biopsy of lung adenocarcinoma: all tumour cells are stained with 5A4 clone (anaplastic lymphoma kinase (ALK) immunohistochemistry (IHC)) original magnification a) ×40 and b) ×200 (Ventana, Tucson, AZ, USA); c) split signals are observed...
Transthoracic needle biopsy of lung adenocarcinoma: all tumour cells are stained with 5A4 clone (anaplastic lymphoma kinase (ALK) immunohistochemistry (IHC)) original magnification a) ×40 and b) ×200 (Ventana, Tucson, AZ, USA); c) split signals are observed in >15% of the tumour cells by fluorescence in situ hybridisation (FISH) using break-apart probe. d) Biopsy of another patient with adenocarcinoma, where nearly all tumour cells are all stained by D4D6 clone (ROS1 IHC Ventana; original magnification ×200); e) split signals are observed in >15% of the tumour cells by FISH using break-apart probe (ZytoVision, Bremerhaven, Germany).
Lukas Bubendorf et al. Eur Respir Rev 2017;26:170007
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