1. Predictive biomarker analyses in cytological specimens
Predictive biomarker analyses in cytological specimens. a) Anaplastic lymphoma kinase (ALK) immunohistochemistry (IHC) on a formalin-fixed paraffin-embedded cell block and b) corresponding ethanol-fixed and previously Papanicolaou-stained cytological smear ...
Predictive biomarker analyses in cytological specimens. a) Anaplastic lymphoma kinase (ALK) immunohistochemistry (IHC) on a formalin-fixed paraffin-embedded cell block and b) corresponding ethanol-fixed and previously Papanicolaou-stained cytological smear of a pulmonary adenocarcinoma (pleural effusion; Leica 5A4 antibody (Leica Biosystems, Newcastle upon Tyne, UK) on Ventana Benchmark XT (Tucson, AZ, USA) and Leica Bondmax automated immunostainers, respectively). c) ALK rearrangement shown by fluorescence in situ hybridisation (FISH) (two single red signals without corresponding green signals and two normal fusion signals per cell nucleus; break-apart FISH probe (Abbott Molecular, Abbott Park, IL, USA)). d) Papanicolaou-stained smear of ROS1-positive adenocarcinoma and e) corresponding ROS1 IHC (pleural effusion; Cell Signaling D4D6 antibody (Danvers, MA, USA) on Leica Bondmax automated immunostainer). f) Ethanol-fixed and previously Papanicolaou-stained smear with membranous and cytoplasmic positivity for programmed death ligand-1 positivity in a fraction of tumour cells (endoscopic ultrasound guided transbronchial fine-needle aspiration; Ventana SP142 antibody on Leica Bondmax automated immunostainer). a), b), d), e), f) Original magnification ×400; c) original magnification ×1000.
Lukas Bubendorf et al. Eur Respir Rev 2017;26:170007
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