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MET and EGFR Mutations Identified in ALK-Rearranged Pulmonary Adenocarcinoma: Molecular Analysis of 25 ALK-Positive Cases  Jennifer M. Boland, MD, Jin.

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Presentation on theme: "MET and EGFR Mutations Identified in ALK-Rearranged Pulmonary Adenocarcinoma: Molecular Analysis of 25 ALK-Positive Cases  Jennifer M. Boland, MD, Jin."— Presentation transcript:

1 MET and EGFR Mutations Identified in ALK-Rearranged Pulmonary Adenocarcinoma: Molecular Analysis of 25 ALK-Positive Cases  Jennifer M. Boland, MD, Jin Sung Jang, PhD, Jun Li, MD, Adam M. Lee, MD, PhD, Jason A. Wampfler, BS, Michele R. Erickson- Johnson, MS, Ibere Soares, MD, Ping Yang, MD, PhD, Jin Jen, MD, PhD, Andre M. Oliveira, MD, PhD, Eunhee S. Yi, MD  Journal of Thoracic Oncology  Volume 8, Issue 5, Pages (May 2013) DOI: /JTO.0b013e318287c395 Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions

2 FIGURE 1 Pulmonary adenocarcinoma (A, hematoxylin and eosin stain; original magnification, 200×) with acinar and micropapillary growth, showing EGFR del L747-S752 by LuCaMSP (B) confirmed by sequencing (C). The tumor shows 1+ ALK staining by immunohistochemistry (D). Fluorescent in situ hybridization (FISH, E) shows a combination of two positive patterns: break-apart of the probes flanking the ALK locus by FISH (arrow), and one fusion with an isolated red signal (arrowhead). Sanger sequencing confirmed the presence of three EML4–ALK transcripts (F), with exon 13 (variant 1) or exon 6 (variant 3a/b) of EML4 fused to exon 20 of ALK. Journal of Thoracic Oncology 2013 8, DOI: ( /JTO.0b013e318287c395) Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions

3 FIGURE 2 Pulmonary adenocarcinoma (A, hematoxylin and eosin stain; original magnification, 200×) with acinar growth and signet ring cells, showing MET N375S by LuCaMSP (B) confirmed by sequencing (C). The tumor shows 3+ ALK staining by immunohistochemistry (D) and break-apart of the probes flanking the ALK locus by fluorescent in situ hybridization (E, arrow). Journal of Thoracic Oncology 2013 8, DOI: ( /JTO.0b013e318287c395) Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions

4 FIGURE 3 Pulmonary adenocarcinoma (A, hematoxylin and eosin stain; original magnification, 200×) with cribriform and solid growth and signet ring cells, showing MET R988C by LuCaMSP (B) confirmed by sequencing (C). The tumor shows 3+ ALK staining by immunohistochemistry (D) and break-apart of the probes flanking the ALK locus by fluorescent in situ hybridization (E, arrow). Journal of Thoracic Oncology 2013 8, DOI: ( /JTO.0b013e318287c395) Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions

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