1. Wednesday Agenda: Pre-Lab Quiz
Lab Day 1 Procedures – Incubation Temperature Difference
HW: Day 1 Post Lab, Read for Day 2
Wednesday

2. Thursday Agenda: STEM Warm-Up Notes: Cloning 20.3 Lab Day 2 Procedures
Lab Write-Up Due March 4th
Thursday

3. Thursday Agenda: Warm-Up Notes: Cloning 20.3
List of BioTech Cons: BioTech Agriculture, Medical, and Industrial
Gel Electrophoresis Notes
Lab Write-Up Due March 4th
Thursday

4. Order for the remainder of the lab:
Post Lab Questions Day 1 – Last night
Day 2 Equations and Pictures
Day 2 Post Lab Questions
Conclusion: Summarize, Error, Apply

5. CLoning
Plants – Totipotent Cells – Can give rise to all cells of the plant (Cutting)
Animals – nuclear potential to differentiate is restricted as the organism ages
Many different types of animals have been cloned since Dolly. What are some conclusions that have been drawn from cloning of animals?
They don’t always look and act identical to their donor. Environmental impacts and random phenomena – mostly due to epigenetic changes of chromatin.

6. Cloning and Stem Cells
Where do they come from?
Which type is better?
Possible therapeutic uses?
Latest: Learned to deprogram differentiated cells and then reprogram them

8. Rat Heart

10. BT Corn Bacillus thuringiensis and European Corn Borer
Bt protein ingested
Binds to the gut wall, breaks down
Insect dies of septicemia as bacteria multiply in the blood
Kills beneficial insects
Humans?

11. Problems with GMOs

12. Around the world 38 countries that have banned GMOs – unknown risks
Some countries have limits - # hectares
Rules on importation, trade arguments
Many GM Labeling Required

14. Lab Discussion
What are some factors that are essential for bacteria to grow?
Inhibit growth?

15. Lab Discussion and predictions
LB - Plasmid LB + Plasmid LB/AMP – Plasmid LB/AMP + Plasmid

16. Friday STEM Agenda: G.M. O? Create GMO Con List
Gel Electrophoresis Notes
Socrative Quiz
HW: PreLab 9
Agenda:
Socrative Quiz
Lab Discussion
Finish Lab 8
Friday

17. Order for the remainder of the lab:
Post Lab Questions Day 1 – Last night
Day 2 Equations and Pictures
Day 2 Post Lab Questions
Conclusion: Summarize, Error, Apply

18. STRs (Short tandem Repeats)
FBI: DNA: 1988
Highly variable areas of our DNA, person to person (2 to 5 nucleotides)
Vary at same locus on different homologs
ACTA 30 times
ACTA 15 times
30, 15
Number of repeats can be identified using Gel Electrophoresis

19. Gel electrophoresis
What is it?
Genetics Utah Animation

20. Standard HindIII Lambda DNA Sample
Ladder or marker is run to show known fragments for comparison.

21. Standard Curve (plot of distance migrated vs. DNA size (base pairs)

22. Practice
A bloody crime scene has occurred, and police have collected several blood samples. The following table shows the number of repeats for three STR markers from those samples. (Normally there are 13 STR markers)
Analyze the data and determine which suspect you would charge with the crime.
Source of Sample
STR Marker 1
STR Marker 2
STR Marker 3
Blood at Crime Scene
12, 15, 18, 19
9, 14, 17
10, 15, 16, 20
Victim
15, 19
9, 17
15, 20
Suspect 1
12, 21
15, 17
10, 18
Suspect 2
12, 18
14, 17
10, 16

24. Lab 9 Restriction Enzymes and DNA
Problem: How can DNA fragments be separated and analyzed by gel electrophoresis?
Restriction enzymes (RE’s): recognize specific DNA sequences and cut DNA at these sites
RE’s in this lab: EcoRI and HindIII
EcoRI: sequence GAATTC, cuts G | AATTC
HindIII: sequence AAGCTT, cuts A | AGCTT
RE’s cut DNA so that a gel electrophoresis can be run to create a DNA fingerprint

25. Friday Homework: Lab 9 PreLab: PreLab Quiz Monday (Set up and safety)
Introduction: One-two paragraphs of key terms
Pre-Lab Questions 1-5
List Materials Day One and Day Two
Safety SAFETY S A F E T Y
Day 1 Procedures (Five activities to read over)
PreLab Quiz Monday (Set up and safety)
Lab 8 and 9 Due: March 4th

26. Monday 2/29/16 Pre-Lab Quiz Complete Lab Activities 1-5
Homework: Work on Labs, Read Day 2 Procedures
Monday 2/29/16

27. Run your gel.
Work on Review Questions for Biotechnology
Go over Gene Regulation Exam
Post Lab Questions, Post Lab Ideal Gel Analysis
Tuesday March 1st