1. Good Morning! Pass your molecule models forward! Warm Up: Today:
List the four macromolecules, their monomers, functions, and an example.
Today:
Enzyme notes (need your own paper)
Video
Worksheet
QUIZ (DNA Electrophoresis)

2. Enzymes

3. What Are Enzymes? Most enzymes are Proteins
Act as Catalyst to accelerate a reaction
Not permanently changed in the process

4. Enzymes Are specific for what they will catalyze Are Reusable
End in –ase
-Sucrase
-Lactase
-Maltase

5. How do enzymes Work?
Enzymes work by weakening bonds which lowers activation energy

6. Enzymes Without Enzyme With Enzyme Free Energy
Progress of the reaction
Reactants
Products
Free energy of activation

7. Enzyme-Substrate Complex
The substance (reactant) an enzyme acts on is the substrate
Enzyme
Joins
Substrate

8. Active Site
A restricted region of an enzyme molecule which binds to the substrate.
Active Site
Enzyme
Substrate

9. Factors Affecting Enzyme Activity
Temperature pH
Concentration
Inhibitors

10. Temperature & pH
High temperatures are the most dangerous reactions & denature enzymes (Most like normal Body temperatures)
Most enzymes like near neutral pH (6 to 8)
Denatured (unfolded) by ionic salts

11. Two examples of Enzyme Inhibitors
a. Competitive inhibitors: are chemicals that resemble an enzyme’s normal substrate and compete with it for the active site.
Enzyme
Substrate
Competitive inhibitor

12. Inhibitors b. Noncompetitive inhibitors:
Inhibitors that do not enter the active site, but bind to another part of the enzyme causing the enzyme to change its shape, which in turn alters the active site.
Enzyme
Noncompetitive
Inhibitor
Substrate
active site
altered

13. S PECIFIC
C ATALYZE
U NCHANGED
R EUSABLE
L OCK AND KEY FIT