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Fig. 1. Ishibashi et al. Leader peptide Mature peptide

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1 Fig. 1. Ishibashi et al. Leader peptide Mature peptide
LaqA MKELSEKELRECVGG SIWGDIGQGVGKAAYWVGKAMGNMSDVNQASRINRKKKH *************** * ***** * ************************** LagA MKELSEKELRECVGG GTWDDIGQGIGRVAYWVGKAMGNMSDVNQASRINRKKKH LaqB MKNNNNNFFKDMEIIEDQELVSITGG KKWGWLAWVEPAGEFLKGFGKGAIKEGNKDKWKNI ** ******* *************** ********* ** ** ******************* LagB MK-NNNNFFKGMEIIEDQELVSITGG KKWGWLAWVDPAYEFIKGFGKGAIKEGNKDKWKNI Fig. 1. Ishibashi et al.

2 Receptor of lactococcin Q
0. 5 kb laqA (orf1) laqB (orf2) laqC (orf3) laqD (orf4) laqE (orf5) lagA lagB lagC lagD lagE (a) (b) LaqD LaqE LaqC Lactococcin Qα Lactococcin Qβ laqA laqB laqC laqD laqE Transcription and translation In Out Receptor of lactococcin Q Secretion Self-immunity PED ABD (c) Fig. 2. Ishibashi et al.

3 (a) (b) Fig. 3. Ishibashi et al. (I) (II) laqC laqD laqE laqA laqB (I)
(kb) 3.0 2.0 1.0 (I) C N P M (II) laqC laqD laqE laqA laqB (I) (II) 1.4 kb 2.2 kb Fig. 3. Ishibashi et al.

4 LaqD 1-(130)-131 FPKPKAEYKPSIERVDGLSSFIPILIKQKSLIFHTALASLFITIFGIISS 180
**************** ** * ******* ************ LagD 1-(130)-131 FPKPKAEYKPSIERVDSLSTFFPILIKQK SLFITIFGIISS 171 LaqD YYFQGLLDNIIPNQARSTLNILSIGLIFVYLFRVLFEYSRSYLLLLIGQR 230-(481)-712 ************************************************** LagD YYFQGLLDNIIPNQARSTLNILSIGLIFVYLFRVLFEYSRSYLLLLIGQR 221-(481)-703 Fig. 4. Ishibashi et al.

5 Fig. 5. Ishibashi et al. - pLaqA-C pLaqC pLaqA-D pLaqA-E pMG36c
PstI SphI pLaqA-C pLaqC pLaqA-D pLaqA-E pMG36c Plasmid pLagA-C-LaqDE pLagC pLaqA-E-d pLagA-C-LaqDE-d Bac Imm LaqQ LaqG - + P32 laqA laqB laqC laqD laqE lagA lagB lagC pLagA-C pLaqABCE Fig. 5. Ishibashi et al.

6 Fig. 6. Ishibashi et al. IL1403 5803 QU 4 pLaqA-E pLagA-C-LaqDE
pLagA-C-LaqDE-d Fig. 6. Ishibashi et al.

7 36c QU 4 LaqC QU 4 LagC QU 4 Fig. 7. Ishibashi et al.

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